While the role of dietary fats in breast cancer remains controversial, the recent cloning of peroxisome proliferator-activated receptor gamma (PPARγ), a nuclear hormone receptor, from human breast cancer cells lines provides a potential molecular link. Several fatty acids from four classes of dietary fats were tested for their ability to mediate the transcriptional activity of PPARγ in MCF-7 and MDA-MB-231 cells using growth media with minimal serum. Whereas omega-3 fatty acids inhibit transactivation of PPARγ to levels below control, omega-6, monounsaturated and saturated fatty acids stimulate the activity of the transcriptional reporter. These studies indicate that individual fatty acids differentially regulate the transcriptional activity of PPARγ by selectively acting as agonists or antagonists. Furthermore, the transcriptional activation of PPARγ correlates with cell proliferation in MCF-7 cells. Understanding the effects of individual fats on breast cancer cells and PPARγ transactivation could provide important new insights into the epidemiology of breast cancer and the role of dietary fat. Copyright (C) 2000 Elsevier Science Ireland Ltd.
|Number of pages||7|
|Journal||Molecular and Cellular Endocrinology|
|State||Published - Feb 25 2000|
Bibliographical noteFunding Information:
This work was supported by a grant from the Breast Cancer Research Program (DAMD17-97-1-7248 to M.W. Kilgore) and grants from the Greenville Hospital/Clemson University Cooperative Research and Education Program (to M.W. Kilgore and T.M. Price). We would like to thank Regina Brierley for technical assistance, Dr Wen Chen for critical review and helpful comments and Dr Herman Senter for statistical analysis.
- Breast cancer
- Dietary fats
- Omega-3 fatty acids
- Omega-6 fatty acids
ASJC Scopus subject areas
- Molecular Biology