TY - JOUR
T1 - Differentiating gold nanorod samples using particle size and shape distributions from transmission electron microscope images
AU - Grulke, Eric A.
AU - Wu, Xiaochun
AU - Ji, Yinglu
AU - Buhr, Egbert
AU - Yamamoto, Kazuhiro
AU - Woong Song, Nam
AU - Stefaniak, Aleksandr B.
AU - Schwegler-Berry, Diane
AU - Burchett, Woodrow W.
AU - Lambert, Joshua
AU - Stromberg, Arnold J.
N1 - Publisher Copyright:
© 2018 BIPM & IOP Publishing Ltd.
PY - 2018/2/28
Y1 - 2018/2/28
N2 - Size and shape distributions of gold nanorod samples are critical to their physico-chemical properties, especially their longitudinal surface plasmon resonance. This interlaboratory comparison study developed methods for measuring and evaluating size and shape distributions for gold nanorod samples using transmission electron microscopy (TEM) images. The objective was to determine whether two different samples, which had different performance attributes in their application, were different with respect to their size and/or shape descriptor distributions. Touching particles in the captured images were identified using a ruggedness shape descriptor. Nanorods could be distinguished from nanocubes using an elongational shape descriptor. A non-parametric statistical test showed that cumulative distributions of an elongational shape descriptor, that is, the aspect ratio, were statistically different between the two samples for all laboratories. While the scale parameters of size and shape distributions were similar for both samples, the width parameters of size and shape distributions were statistically different. This protocol fulfills an important need for a standardized approach to measure gold nanorod size and shape distributions for applications in which quantitative measurements and comparisons are important. Furthermore, the validated protocol workflow can be automated, thus providing consistent and rapid measurements of nanorod size and shape distributions for researchers, regulatory agencies, and industry.
AB - Size and shape distributions of gold nanorod samples are critical to their physico-chemical properties, especially their longitudinal surface plasmon resonance. This interlaboratory comparison study developed methods for measuring and evaluating size and shape distributions for gold nanorod samples using transmission electron microscopy (TEM) images. The objective was to determine whether two different samples, which had different performance attributes in their application, were different with respect to their size and/or shape descriptor distributions. Touching particles in the captured images were identified using a ruggedness shape descriptor. Nanorods could be distinguished from nanocubes using an elongational shape descriptor. A non-parametric statistical test showed that cumulative distributions of an elongational shape descriptor, that is, the aspect ratio, were statistically different between the two samples for all laboratories. While the scale parameters of size and shape distributions were similar for both samples, the width parameters of size and shape distributions were statistically different. This protocol fulfills an important need for a standardized approach to measure gold nanorod size and shape distributions for applications in which quantitative measurements and comparisons are important. Furthermore, the validated protocol workflow can be automated, thus providing consistent and rapid measurements of nanorod size and shape distributions for researchers, regulatory agencies, and industry.
KW - gold nanorod
KW - shape distribution
KW - size distribution
KW - transmission electron microscopy
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U2 - 10.1088/1681-7575/aaa368
DO - 10.1088/1681-7575/aaa368
M3 - Article
AN - SCOPUS:85045642221
SN - 0026-1394
VL - 55
SP - 254
EP - 267
JO - Metrologia
JF - Metrologia
IS - 2
ER -