Differentiation of Neospora hughesi from Neospora caninum based on their immunodominant surface antigen, SAG1 and SRS2

A. E. Marsh; D. K. Howe; G. Wang; B. C. Barr; N. Cannon; P. A. Conrad

doi:10.1016/S0020-7519(99)00120-4

Differentiation of Neospora hughesi from Neospora caninum based on their immunodominant surface antigen, SAG1 and SRS2

A. E. Marsh, D. K. Howe, G. Wang, B. C. Barr, N. Cannon, P. A. Conrad

Research output: Contribution to journal › Article › peer-review

68 Scopus citations

Abstract

Neospora hughesi is a newly recognised parasite that is closely related to Neospora caninum, and is a cause of equine protozoal myeloencephalitis. We have characterised two N. hughesi immunodominant tachyzoite antigens which exhibit antigenic and molecular differences from the homologous tachyzoite antigens on N. caninum. These antigens on N. hughesi are referred to as NhSAG1 and NhSRS2, using the same mnemonics as used for the N. caninum antigens (NcSAG1 and NcSRS2), and are homologous to Toxoplasma gondii surface antigen 1 (SAG1) and SAG1-related sequence 2 (SRS2). The NcSAG1 and NcSRS2 were antigenically conserved in six different N. caninum isolates from cattle and dogs. The two equine-derived Neospora isolates, one designated as N. hughesi, were similar to each other but different from N. caninum. There was 6% difference in amino acid identity between NcSAG1 and NhSAG1, whereas there was a 9% difference when NcSRS2 and NhSRS2 were compared. The polymorphism of these genes and their corresponding proteins provide additional markers which can be used to distinguish N. caninum from N. hughesi.

Original language	English
Pages (from-to)	1575-1582
Number of pages	8
Journal	International Journal for Parasitology
Volume	29
Issue number	10
DOIs	https://doi.org/10.1016/S0020-7519(99)00120-4
State	Published - Oct 1999

Bibliographical note

Funding Information:
This project was supported by the Grayson Jockey Club Research Foundation, Lexington, Kentucky, and the Center for Equine Health, University of California, Davis with funds provided by the Oak Tree Racing Association, the state of California satellite wagering fund, and contributions from private donors. Partial support provided by the USDA National Research Initiative (97-35204-4770) to D.K.H. (Dr Sibley). We thank Dr Byron Blagburn for providing the Alabama equine Neospora isolate and Dr David Sibley for his thoughtful comments and review of this study.

Keywords

Equine protozoal myeloencephalitis
Neospora caninum
Neospora hughesi
Tachyzoite surface antigen

ASJC Scopus subject areas

Parasitology
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/S0020-7519(99)00120-4

Cite this

@article{a21ca6fbf6f242c9b747b858621283dd,

title = "Differentiation of Neospora hughesi from Neospora caninum based on their immunodominant surface antigen, SAG1 and SRS2",

abstract = "Neospora hughesi is a newly recognised parasite that is closely related to Neospora caninum, and is a cause of equine protozoal myeloencephalitis. We have characterised two N. hughesi immunodominant tachyzoite antigens which exhibit antigenic and molecular differences from the homologous tachyzoite antigens on N. caninum. These antigens on N. hughesi are referred to as NhSAG1 and NhSRS2, using the same mnemonics as used for the N. caninum antigens (NcSAG1 and NcSRS2), and are homologous to Toxoplasma gondii surface antigen 1 (SAG1) and SAG1-related sequence 2 (SRS2). The NcSAG1 and NcSRS2 were antigenically conserved in six different N. caninum isolates from cattle and dogs. The two equine-derived Neospora isolates, one designated as N. hughesi, were similar to each other but different from N. caninum. There was 6% difference in amino acid identity between NcSAG1 and NhSAG1, whereas there was a 9% difference when NcSRS2 and NhSRS2 were compared. The polymorphism of these genes and their corresponding proteins provide additional markers which can be used to distinguish N. caninum from N. hughesi.",

keywords = "Equine protozoal myeloencephalitis, Neospora caninum, Neospora hughesi, Tachyzoite surface antigen",

author = "Marsh, {A. E.} and Howe, {D. K.} and G. Wang and Barr, {B. C.} and N. Cannon and Conrad, {P. A.}",

note = "Funding Information: This project was supported by the Grayson Jockey Club Research Foundation, Lexington, Kentucky, and the Center for Equine Health, University of California, Davis with funds provided by the Oak Tree Racing Association, the state of California satellite wagering fund, and contributions from private donors. Partial support provided by the USDA National Research Initiative (97-35204-4770) to D.K.H. (Dr Sibley). We thank Dr Byron Blagburn for providing the Alabama equine Neospora isolate and Dr David Sibley for his thoughtful comments and review of this study. ",

year = "1999",

month = oct,

doi = "10.1016/S0020-7519(99)00120-4",

language = "English",

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pages = "1575--1582",

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TY - JOUR

T1 - Differentiation of Neospora hughesi from Neospora caninum based on their immunodominant surface antigen, SAG1 and SRS2

AU - Marsh, A. E.

AU - Howe, D. K.

AU - Wang, G.

AU - Barr, B. C.

AU - Cannon, N.

AU - Conrad, P. A.

N1 - Funding Information: This project was supported by the Grayson Jockey Club Research Foundation, Lexington, Kentucky, and the Center for Equine Health, University of California, Davis with funds provided by the Oak Tree Racing Association, the state of California satellite wagering fund, and contributions from private donors. Partial support provided by the USDA National Research Initiative (97-35204-4770) to D.K.H. (Dr Sibley). We thank Dr Byron Blagburn for providing the Alabama equine Neospora isolate and Dr David Sibley for his thoughtful comments and review of this study.

PY - 1999/10

Y1 - 1999/10

N2 - Neospora hughesi is a newly recognised parasite that is closely related to Neospora caninum, and is a cause of equine protozoal myeloencephalitis. We have characterised two N. hughesi immunodominant tachyzoite antigens which exhibit antigenic and molecular differences from the homologous tachyzoite antigens on N. caninum. These antigens on N. hughesi are referred to as NhSAG1 and NhSRS2, using the same mnemonics as used for the N. caninum antigens (NcSAG1 and NcSRS2), and are homologous to Toxoplasma gondii surface antigen 1 (SAG1) and SAG1-related sequence 2 (SRS2). The NcSAG1 and NcSRS2 were antigenically conserved in six different N. caninum isolates from cattle and dogs. The two equine-derived Neospora isolates, one designated as N. hughesi, were similar to each other but different from N. caninum. There was 6% difference in amino acid identity between NcSAG1 and NhSAG1, whereas there was a 9% difference when NcSRS2 and NhSRS2 were compared. The polymorphism of these genes and their corresponding proteins provide additional markers which can be used to distinguish N. caninum from N. hughesi.

AB - Neospora hughesi is a newly recognised parasite that is closely related to Neospora caninum, and is a cause of equine protozoal myeloencephalitis. We have characterised two N. hughesi immunodominant tachyzoite antigens which exhibit antigenic and molecular differences from the homologous tachyzoite antigens on N. caninum. These antigens on N. hughesi are referred to as NhSAG1 and NhSRS2, using the same mnemonics as used for the N. caninum antigens (NcSAG1 and NcSRS2), and are homologous to Toxoplasma gondii surface antigen 1 (SAG1) and SAG1-related sequence 2 (SRS2). The NcSAG1 and NcSRS2 were antigenically conserved in six different N. caninum isolates from cattle and dogs. The two equine-derived Neospora isolates, one designated as N. hughesi, were similar to each other but different from N. caninum. There was 6% difference in amino acid identity between NcSAG1 and NhSAG1, whereas there was a 9% difference when NcSRS2 and NhSRS2 were compared. The polymorphism of these genes and their corresponding proteins provide additional markers which can be used to distinguish N. caninum from N. hughesi.

KW - Equine protozoal myeloencephalitis

KW - Neospora caninum

KW - Neospora hughesi

KW - Tachyzoite surface antigen

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U2 - 10.1016/S0020-7519(99)00120-4

DO - 10.1016/S0020-7519(99)00120-4

M3 - Article

C2 - 10608444

AN - SCOPUS:0032724588

SN - 0020-7519

VL - 29

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EP - 1582

JO - International Journal for Parasitology

JF - International Journal for Parasitology

IS - 10

ER -

Differentiation of Neospora hughesi from Neospora caninum based on their immunodominant surface antigen, SAG1 and SRS2

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

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