Diffuse reflectance spectroscopy for optical characterizations of orthotopic head and neck cancer models in vivo

Pranto Soumik Saha, Jing Yan, Caigang Zhu

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

We demonstrated an easy-to-build, portable diffuse reflectance spectroscopy device along with a Monte Carlo inverse model to quantify tissue absorption and scattering-based parameters of orthotopic head and neck cancer models in vivo. Both tissue-mimicking phantom studies and animal studies were conducted to verify the optical spectroscopy system and Monte Carlo inverse model for the accurate extraction of tissue optical properties. For the first time, we reported the tissue absorption and scattering coefficients of mouse normal tongue tissues and tongue tumor tissues. Our in vivo animal studies showed reduced total hemoglobin concentration, lower tissue vascular oxygen saturation, and increased tissue scattering in the orthotopic tongue tumors compared to the normal tongue tissues. Our data also showed that mice tongue tumors with different sizes may have significantly different tissue absorption and scattering-based parameters. Small tongue tumors (volume was ∼60 mm3) had increased absorption coefficients, decreased reduced-scattering coefficients, and increased total hemoglobin concentrations compared to tiny tongue tumors (volume was ∼18 mm3). These results demonstrated the potential of diffuse reflectance spectroscopy to noninvasively evaluate tumor biology using orthotopic tongue cancer models for future head and neck cancer research.

Original languageEnglish
Pages (from-to)4176-4189
Number of pages14
JournalBiomedical Optics Express
Volume15
Issue number7
DOIs
StatePublished - Jul 1 2024

Bibliographical note

Publisher Copyright:
© 2024 Optica Publishing Group under the terms of the Optica Open Access Publishing Agreement.

ASJC Scopus subject areas

  • Biotechnology
  • Atomic and Molecular Physics, and Optics

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