Diffusion-mediated production of protein gradients by way of variable depth hydrogel microstamps

Dewayne L. Thompson; Ashley E. Madon; Christine A. Trinkle

doi:10.1115/IMECE2009-11292

Diffusion-mediated production of protein gradients by way of variable depth hydrogel microstamps

Dewayne L. Thompson, Ashley E. Madon, Christine A. Trinkle

Mechanical and Aerospace Engineering

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

The precise application of proteins and other biomolecules to create patterned surfaces is an important step in many processes, including the creation of biosensors and directed cell growth for tissue engineering [1,2]. While traditional poly-dimethylsiloxane (PDMS) elastomeric stamps have been used to successfully transfer proteins with submicron resolution, the resulting patterns are limited to a single, uniform protein concentration. The technique presented here utilizes varied-topography hydrophilic stamps as a diffusion medium, allowing protein gradients to be easily applied and accurately reproduced. Specifically, stamps of a 2% agarose hydrogel are used to demonstrate variable-concentration patterning of a fluorescently-labeled protein.

Original language	English
Title of host publication	Proceedings of the ASME International Mechanical Engineering Congress and Exposition 2009, IMECE 2009
Pages	255-256
Number of pages	2
DOIs	https://doi.org/10.1115/IMECE2009-11292
State	Published - 2010
Event	2009 ASME International Mechanical Engineering Congress and Exposition, IMECE2009 - Lake Buena Vista, FL, United States Duration: Nov 13 2009 → Nov 19 2009

Publication series

Name	ASME International Mechanical Engineering Congress and Exposition, Proceedings
Volume	2

Conference

Conference	2009 ASME International Mechanical Engineering Congress and Exposition, IMECE2009
Country/Territory	United States
City	Lake Buena Vista, FL
Period	11/13/09 → 11/19/09

ASJC Scopus subject areas

Mechanical Engineering

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10.1115/IMECE2009-11292

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Thompson, D. L., Madon, A. E., & Trinkle, C. A. (2010). Diffusion-mediated production of protein gradients by way of variable depth hydrogel microstamps. In Proceedings of the ASME International Mechanical Engineering Congress and Exposition 2009, IMECE 2009 (pp. 255-256). (ASME International Mechanical Engineering Congress and Exposition, Proceedings; Vol. 2). https://doi.org/10.1115/IMECE2009-11292

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title = "Diffusion-mediated production of protein gradients by way of variable depth hydrogel microstamps",

abstract = "The precise application of proteins and other biomolecules to create patterned surfaces is an important step in many processes, including the creation of biosensors and directed cell growth for tissue engineering [1,2]. While traditional poly-dimethylsiloxane (PDMS) elastomeric stamps have been used to successfully transfer proteins with submicron resolution, the resulting patterns are limited to a single, uniform protein concentration. The technique presented here utilizes varied-topography hydrophilic stamps as a diffusion medium, allowing protein gradients to be easily applied and accurately reproduced. Specifically, stamps of a 2% agarose hydrogel are used to demonstrate variable-concentration patterning of a fluorescently-labeled protein.",

author = "Thompson, {Dewayne L.} and Madon, {Ashley E.} and Trinkle, {Christine A.}",

year = "2010",

doi = "10.1115/IMECE2009-11292",

language = "English",

isbn = "9780791843758",

series = "ASME International Mechanical Engineering Congress and Exposition, Proceedings",

pages = "255--256",

booktitle = "Proceedings of the ASME International Mechanical Engineering Congress and Exposition 2009, IMECE 2009",

note = "2009 ASME International Mechanical Engineering Congress and Exposition, IMECE2009 ; Conference date: 13-11-2009 Through 19-11-2009",

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Thompson, DL, Madon, AE & Trinkle, CA 2010, Diffusion-mediated production of protein gradients by way of variable depth hydrogel microstamps. in Proceedings of the ASME International Mechanical Engineering Congress and Exposition 2009, IMECE 2009. ASME International Mechanical Engineering Congress and Exposition, Proceedings, vol. 2, pp. 255-256, 2009 ASME International Mechanical Engineering Congress and Exposition, IMECE2009, Lake Buena Vista, FL, United States, 11/13/09. https://doi.org/10.1115/IMECE2009-11292

Diffusion-mediated production of protein gradients by way of variable depth hydrogel microstamps. / Thompson, Dewayne L.; Madon, Ashley E.; Trinkle, Christine A.
Proceedings of the ASME International Mechanical Engineering Congress and Exposition 2009, IMECE 2009. 2010. p. 255-256 (ASME International Mechanical Engineering Congress and Exposition, Proceedings; Vol. 2).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

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N2 - The precise application of proteins and other biomolecules to create patterned surfaces is an important step in many processes, including the creation of biosensors and directed cell growth for tissue engineering [1,2]. While traditional poly-dimethylsiloxane (PDMS) elastomeric stamps have been used to successfully transfer proteins with submicron resolution, the resulting patterns are limited to a single, uniform protein concentration. The technique presented here utilizes varied-topography hydrophilic stamps as a diffusion medium, allowing protein gradients to be easily applied and accurately reproduced. Specifically, stamps of a 2% agarose hydrogel are used to demonstrate variable-concentration patterning of a fluorescently-labeled protein.

AB - The precise application of proteins and other biomolecules to create patterned surfaces is an important step in many processes, including the creation of biosensors and directed cell growth for tissue engineering [1,2]. While traditional poly-dimethylsiloxane (PDMS) elastomeric stamps have been used to successfully transfer proteins with submicron resolution, the resulting patterns are limited to a single, uniform protein concentration. The technique presented here utilizes varied-topography hydrophilic stamps as a diffusion medium, allowing protein gradients to be easily applied and accurately reproduced. Specifically, stamps of a 2% agarose hydrogel are used to demonstrate variable-concentration patterning of a fluorescently-labeled protein.

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BT - Proceedings of the ASME International Mechanical Engineering Congress and Exposition 2009, IMECE 2009

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Diffusion-mediated production of protein gradients by way of variable depth hydrogel microstamps

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