Diolistic labeling and analysis of dendritic spines

M. Foster Olive; Armani P. Del Franco; Cassandra D. Gipson

doi:10.1007/978-1-4939-7571-6_14

Diolistic labeling and analysis of dendritic spines

M. Foster Olive, Armani P. Del Franco, Cassandra D. Gipson

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

11 Scopus citations

Abstract

Dendritic spines are diverse and plastic components of the neuronal cell apparatus and are highly responsive to trophic factors during both development and adulthood. Diolistic labeling of neurons with lipophilic fluorescent dyes, coupled with advanced high-resolution microscopy methods, provides robust labeling of dendritic spines for assessment of their density and morphology. Here, we describe a method for labeling of dendritic spines using diolistic labeling in ex vivo brain slices, visualization using confocal laser scanning microscopy, deconvolution, and analysis using the Surpass module of Bitplane Imaris software.

Original language	English
Title of host publication	Methods in Molecular Biology
Pages	179-200
Number of pages	22
DOIs	https://doi.org/10.1007/978-1-4939-7571-6_14
State	Published - 2018

Publication series

Name	Methods in Molecular Biology
Volume	1727
ISSN (Print)	1064-3745

Bibliographical note

Publisher Copyright:
© Springer Science+Business Media, LLC 2018.

Keywords

3-D reconstruction
Confocal imaging
Deconvolution
Dendritic spine
Diolistic
Lipophilic dye
Morphology

ASJC Scopus subject areas

Molecular Biology
Genetics

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10.1007/978-1-4939-7571-6_14

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AB - Dendritic spines are diverse and plastic components of the neuronal cell apparatus and are highly responsive to trophic factors during both development and adulthood. Diolistic labeling of neurons with lipophilic fluorescent dyes, coupled with advanced high-resolution microscopy methods, provides robust labeling of dendritic spines for assessment of their density and morphology. Here, we describe a method for labeling of dendritic spines using diolistic labeling in ex vivo brain slices, visualization using confocal laser scanning microscopy, deconvolution, and analysis using the Surpass module of Bitplane Imaris software.

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KW - Deconvolution

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KW - Lipophilic dye

KW - Morphology

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Diolistic labeling and analysis of dendritic spines

Abstract

Publication series

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

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