Diolistic labeling and analysis of dendritic spines

M. Foster Olive, Armani P. Del Franco, Cassandra D. Gipson

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

9 Scopus citations


Dendritic spines are diverse and plastic components of the neuronal cell apparatus and are highly responsive to trophic factors during both development and adulthood. Diolistic labeling of neurons with lipophilic fluorescent dyes, coupled with advanced high-resolution microscopy methods, provides robust labeling of dendritic spines for assessment of their density and morphology. Here, we describe a method for labeling of dendritic spines using diolistic labeling in ex vivo brain slices, visualization using confocal laser scanning microscopy, deconvolution, and analysis using the Surpass module of Bitplane Imaris software.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
Number of pages22
StatePublished - 2018

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745

Bibliographical note

Funding Information:
This work was supported by NIH grants DA024355, DA037741, and AA025590 to MFO and NIH grants DA DA036569 to CDG.

Publisher Copyright:
© Springer Science+Business Media, LLC 2018.


  • 3-D reconstruction
  • Confocal imaging
  • Deconvolution
  • Dendritic spine
  • Diolistic
  • Lipophilic dye
  • Morphology

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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