Dendritic spines are diverse and plastic components of the neuronal cell apparatus and are highly responsive to trophic factors during both development and adulthood. Diolistic labeling of neurons with lipophilic fluorescent dyes, coupled with advanced high-resolution microscopy methods, provides robust labeling of dendritic spines for assessment of their density and morphology. Here, we describe a method for labeling of dendritic spines using diolistic labeling in ex vivo brain slices, visualization using confocal laser scanning microscopy, deconvolution, and analysis using the Surpass module of Bitplane Imaris software.
|Title of host publication||Methods in Molecular Biology|
|Number of pages||22|
|State||Published - 2018|
|Name||Methods in Molecular Biology|
Bibliographical noteFunding Information:
This work was supported by NIH grants DA024355, DA037741, and AA025590 to MFO and NIH grants DA DA036569 to CDG.
© Springer Science+Business Media, LLC 2018.
- 3-D reconstruction
- Confocal imaging
- Dendritic spine
- Lipophilic dye
ASJC Scopus subject areas
- Molecular Biology