Direct cloning of double-stranded RNAs

Manli Shen, Marina Falaleeva, Natalia Korotkova, Stefan Stamm

Research output: Contribution to journalArticlepeer-review


Most annotated genomes show a large number of sense-antisense transcripts that can generate doublestranded RNAs. We describe a method to clone these dsRNAs from total RNA preparations.

Original languageEnglish
Pages (from-to)53-64
Number of pages12
JournalMethods in Molecular Biology
StatePublished - 2015

Bibliographical note

Publisher Copyright:
© Springer Science+Business Media New York 2015.


  • Cloning
  • Sense-antisense pair
  • dsRNA

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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