Direct cloning of double-stranded RNAs

Manli Shen; Marina Falaleeva; Natalia Korotkova; Stefan Stamm

doi:10.1007/978-1-4939-2547-6_6

Direct cloning of double-stranded RNAs

Manli Shen, Marina Falaleeva, Natalia Korotkova, Stefan Stamm

Research output: Contribution to journal › Article › peer-review

Abstract

Most annotated genomes show a large number of sense-antisense transcripts that can generate doublestranded RNAs. We describe a method to clone these dsRNAs from total RNA preparations.

Original language	English
Pages (from-to)	53-64
Number of pages	12
Journal	Methods in Molecular Biology
Volume	1296
DOIs	https://doi.org/10.1007/978-1-4939-2547-6_6
State	Published - 2015

Bibliographical note

Publisher Copyright:
© Springer Science+Business Media New York 2015.

Funding

Funders	Funder number
National Institutes of Health (NIH)	GM083187, P20GM1034865-10
National Institute of General Medical Sciences	R01GM083187

Keywords

Cloning
Sense-antisense pair
dsRNA

ASJC Scopus subject areas

Molecular Biology
Genetics

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10.1007/978-1-4939-2547-6_6

Cite this

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title = "Direct cloning of double-stranded RNAs",

abstract = "Most annotated genomes show a large number of sense-antisense transcripts that can generate doublestranded RNAs. We describe a method to clone these dsRNAs from total RNA preparations.",

keywords = "Cloning, Sense-antisense pair, dsRNA",

author = "Manli Shen and Marina Falaleeva and Natalia Korotkova and Stefan Stamm",

note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media New York 2015.",

year = "2015",

doi = "10.1007/978-1-4939-2547-6\_6",

language = "English",

volume = "1296",

pages = "53--64",

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TY - JOUR

T1 - Direct cloning of double-stranded RNAs

AU - Shen, Manli

AU - Falaleeva, Marina

AU - Korotkova, Natalia

AU - Stamm, Stefan

N1 - Publisher Copyright: © Springer Science+Business Media New York 2015.

PY - 2015

Y1 - 2015

N2 - Most annotated genomes show a large number of sense-antisense transcripts that can generate doublestranded RNAs. We describe a method to clone these dsRNAs from total RNA preparations.

AB - Most annotated genomes show a large number of sense-antisense transcripts that can generate doublestranded RNAs. We describe a method to clone these dsRNAs from total RNA preparations.

KW - Cloning

KW - Sense-antisense pair

KW - dsRNA

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UR - http://www.scopus.com/inward/citedby.url?scp=84925297305&partnerID=8YFLogxK

U2 - 10.1007/978-1-4939-2547-6_6

DO - 10.1007/978-1-4939-2547-6_6

M3 - Article

C2 - 25791590

AN - SCOPUS:84925297305

SN - 1064-3745

VL - 1296

SP - 53

EP - 64

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

ER -

Direct cloning of double-stranded RNAs

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

Access to Document

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