Direct Detection of Tobacco Mosaic Virus in Infected Plants with SERS-Sensing Hydrogels

Lyndsay N. Kissell; Huazhen Liu; Manisha Sheokand; Der Vang; Pradeep Kachroo; Pietro Strobbia

doi:10.1021/acssensors.3c02537

Direct Detection of Tobacco Mosaic Virus in Infected Plants with SERS-Sensing Hydrogels

Lyndsay N. Kissell, Huazhen Liu, Manisha Sheokand, Der Vang, Pradeep Kachroo, Pietro Strobbia

Plant Pathology

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

The impact of plant pathogens on global crop yields is a major societal concern. The current agricultural diagnostic paradigm involves either visual inspection (inaccurate) or laboratory molecular tests (burdensome). While field-ready diagnostic methods have advanced in recent years, issues remain with detection of presymptomatic infections, multiplexed analysis, and requirement for in-field sample processing. To overcome these issues, we developed surface-enhanced Raman scattering (SERS)-sensing hydrogels that detect pathogens through simple contact with a leaf. In this work, we developed a novel reagentless SERS sensor for the detection of tobacco mosaic virus (TMV) and embedded it in an optimized hydrogel material to produce sensing hydrogels. To test the diagnostic application of our sensing hydrogels, we demonstrate their use to detect TMV infection in tobacco plants. This technology has the potential to shift the current agricultural diagnostic paradigm by offering a field-deployable tool for presymptomatic and multiplexed molecular identification of pathogens.

Original language	English
Pages (from-to)	514-523
Number of pages	10
Journal	ACS Sensors
Volume	9
Issue number	1
DOIs	https://doi.org/10.1021/acssensors.3c02537
State	Published - Jan 26 2024

Bibliographical note

Publisher Copyright:
© 2024 American Chemical Society

Funding

The authors would like to thank Hope Kumakli and Dr. Ryan White (UC Chemistry) for providing access for electrochemical impedance measurements. We would also like to acknowledge Dr. Tucker Mackenzie and Dr. Neil Aryes (UC Chemistry) for providing access and training on use of the rheometer. We also thank Wendy Havens (UK Plant Pathology) for providing purified TMV and CMV viruses. TEM imaging for this project was made possible using the Cincinnati Children’s Pathology Research Core [RRID#: SCR_022637]. This work was supported by grants from National Science Foundation (IOS#051909, IOS#0817818), and USDA National Institute of Food and Agriculture (GRANT13323564 and Hatch project 1014539). H.L. was supported by a fellowship from Altria Group Inc.

Funders	Funder number
Hope Kumakli	SCR_022637
National Science Foundation (NSF)	0817818, IOS#051909
Altria Group
National Institute of Food and Agriculture	GRANT13323564, 1014539

Keywords

RNA
SERS
hydrogel
in situ
plants
virus

ASJC Scopus subject areas

Bioengineering
Instrumentation
Process Chemistry and Technology
Fluid Flow and Transfer Processes

Access to Document

10.1021/acssensors.3c02537

Cite this

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title = "Direct Detection of Tobacco Mosaic Virus in Infected Plants with SERS-Sensing Hydrogels",

abstract = "The impact of plant pathogens on global crop yields is a major societal concern. The current agricultural diagnostic paradigm involves either visual inspection (inaccurate) or laboratory molecular tests (burdensome). While field-ready diagnostic methods have advanced in recent years, issues remain with detection of presymptomatic infections, multiplexed analysis, and requirement for in-field sample processing. To overcome these issues, we developed surface-enhanced Raman scattering (SERS)-sensing hydrogels that detect pathogens through simple contact with a leaf. In this work, we developed a novel reagentless SERS sensor for the detection of tobacco mosaic virus (TMV) and embedded it in an optimized hydrogel material to produce sensing hydrogels. To test the diagnostic application of our sensing hydrogels, we demonstrate their use to detect TMV infection in tobacco plants. This technology has the potential to shift the current agricultural diagnostic paradigm by offering a field-deployable tool for presymptomatic and multiplexed molecular identification of pathogens.",

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Direct Detection of Tobacco Mosaic Virus in Infected Plants with SERS-Sensing Hydrogels

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

Access to Document

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