Direct in vivo evidence that D2 dopamine receptors can modulate dopamine uptake

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163 Scopus citations

Abstract

In vivo electrochemistry was used to determine the effects of locally applied raclopride (a D2 receptor antagonist) and SCH-23390 (a D1 receptor antagonist) on the clearance of locally applied dopamine in the striatum, nucleus accumbens, and medial prefrontal cortex of rats. Chronoamperometric recordings were continuously made at 5 Hz using Nafion-coated, single carbon fiber electrodes. When a calibrated amount of dopamine was pressure ejected at 5-min intervals from a micropipette adjacent (280-310 μm) to the electrode, transient and reproducible dopamine signals were detected in all three regions. Local application of raclopride from a second micropipette, prior to pressure ejection of dopamine, increased the amplitude and time course of the dopamine signals, indicating significant inhibition of the dopamine transporter. In contrast, local application of SCH-23390 or saline had no effect on the dopamine signals. These data indicate that D2, but not D1, dopamine receptors can modulate the activity of the dopamine transporter.

Original languageEnglish
Pages (from-to)259-263
Number of pages5
JournalNeuroscience Letters
Volume176
Issue number2
DOIs
StatePublished - Aug 1 1994

Bibliographical note

Funding Information:
This work was supported by USPHS Grants AG00441, AG06434 and NS09199, and NSF Grant BNS-9110308T. he authors would like to thank Scott Robinson for constructingth e electrodesu sed in this study and Lihong Diao for technicaal ssistance.

Keywords

  • In vivo electrochemistry
  • Medial prefrontal cortex
  • Nucleus accumbens
  • Raclopride
  • SCH-23390
  • Striatum

ASJC Scopus subject areas

  • General Neuroscience

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