Abstract
The structural changes in fibroblast cell membranes associated with permeability increases due to a chemical injury by saponin and successive Poloxamer 188 (P188) mediated membrane sealing were characterized using atomic force microscope (AFM). In combination with the AFM imaging, a cell viability measurement was also performed. Ethidium homodimer-1 (EH) saponin/water and calcien-AM were used to stain the cells. Direct evidence was obtained that P188 restore the integrity of compromised cell membranes and increase the survival of the treated cells.
| Original language | English |
|---|---|
| Pages (from-to) | 185-186 |
| Number of pages | 2 |
| Journal | MCB Molecular and Cellular Biomechanics |
| Volume | 3 |
| Issue number | 4 |
| State | Published - Dec 2006 |
ASJC Scopus subject areas
- Biophysics
- Molecular Medicine
- Molecular Biology
- Cell Biology