Direct PCR of intact bacteria (colony PCR)

Michael E. Woodman, Christina R. Savage, William K. Arnold, Brian Stevenson

Research output: Contribution to journalArticlepeer-review

28 Scopus citations


This protocol describes an efficient method for screening intact bacteria for the presence of desired DNA sequences using the polymerase chain reaction (PCR). This method is commonly referred to as colony PCR.

Original languageEnglish
Pages (from-to)A.3D.1-A.3D.7
JournalCurrent Protocols in Microbiology
StatePublished - 2016

Bibliographical note

Publisher Copyright:
© 2016 by John Wiley & Sons, Inc.


  • Bacteria
  • PCR
  • screen

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Virology


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