Direct PCR of intact bacteria (colony PCR)

Michael E. Woodman; Christina R. Savage; William K. Arnold; Brian Stevenson

doi:10.1002/cpmc.14

Direct PCR of intact bacteria (colony PCR)

Michael E. Woodman, Christina R. Savage, William K. Arnold, Brian Stevenson

Research output: Contribution to journal › Article › peer-review

36 Scopus citations

Abstract

This protocol describes an efficient method for screening intact bacteria for the presence of desired DNA sequences using the polymerase chain reaction (PCR). This method is commonly referred to as colony PCR.

Original language	English
Pages (from-to)	A.3D.1-A.3D.7
Journal	Current Protocols in Microbiology
Volume	2016
DOIs	https://doi.org/10.1002/cpmc.14
State	Published - 2016

Bibliographical note

Publisher Copyright:
© 2016 by John Wiley & Sons, Inc.

Keywords

Bacteria
PCR
screen

ASJC Scopus subject areas

Parasitology
Microbiology
Virology

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10.1002/cpmc.14

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title = "Direct PCR of intact bacteria (colony PCR)",

abstract = "This protocol describes an efficient method for screening intact bacteria for the presence of desired DNA sequences using the polymerase chain reaction (PCR). This method is commonly referred to as colony PCR.",

keywords = "Bacteria, PCR, screen",

author = "Woodman, {Michael E.} and Savage, {Christina R.} and Arnold, {William K.} and Brian Stevenson",

note = "Publisher Copyright: {\textcopyright} 2016 by John Wiley & Sons, Inc.",

year = "2016",

doi = "10.1002/cpmc.14",

language = "English",

volume = "2016",

pages = "A.3D.1--A.3D.7",

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AU - Woodman, Michael E.

AU - Savage, Christina R.

AU - Arnold, William K.

AU - Stevenson, Brian

PY - 2016

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N2 - This protocol describes an efficient method for screening intact bacteria for the presence of desired DNA sequences using the polymerase chain reaction (PCR). This method is commonly referred to as colony PCR.

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KW - PCR

KW - screen

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VL - 2016

SP - A.3D.1-A.3D.7

JO - Current Protocols in Microbiology

JF - Current Protocols in Microbiology

ER -

Direct PCR of intact bacteria (colony PCR)

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

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