TY - JOUR
T1 - Direct quantification of AD-36 adenovirus DNA by capillary electrophoresis with laser-induced fluorescence
AU - Kolesar, Jill M.
AU - Miller, Judith A.
AU - Dhurandhar, Nikhil V.
AU - Atkinson, Richard L.
PY - 2000/7/7
Y1 - 2000/7/7
N2 - An adenovirus, AD-36, has been linked to human adiposity and a sensitive and reliable quantitative method is required to assess AD-36 viral loads. This report describes direct detection of AD-36 viral DNA, which is the first method to quantitate DNA without amplification. Total genomic DNA is hybridized with an AD-36 specific fluorescently labeled probe and analyzed by capillary electrophoresis with laser-induced fluorescence. The minimum detectable quantity is 10.3 ng/ml, corresponding to 282 copies of AD-36 with a precision of 1-6%. These results indicate that direct detection with capillary electrophoresis with laser-induced fluorescence (CE-LIF) is a reliable and sensitive method for quantifying AD-36 viral DNA. Copyright (C) 2000 Elsevier Science B.V.
AB - An adenovirus, AD-36, has been linked to human adiposity and a sensitive and reliable quantitative method is required to assess AD-36 viral loads. This report describes direct detection of AD-36 viral DNA, which is the first method to quantitate DNA without amplification. Total genomic DNA is hybridized with an AD-36 specific fluorescently labeled probe and analyzed by capillary electrophoresis with laser-induced fluorescence. The minimum detectable quantity is 10.3 ng/ml, corresponding to 282 copies of AD-36 with a precision of 1-6%. These results indicate that direct detection with capillary electrophoresis with laser-induced fluorescence (CE-LIF) is a reliable and sensitive method for quantifying AD-36 viral DNA. Copyright (C) 2000 Elsevier Science B.V.
KW - AD-36 Adenovirus
KW - DNA
KW - Gene quantification
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U2 - 10.1016/S0378-4347(00)00219-X
DO - 10.1016/S0378-4347(00)00219-X
M3 - Article
C2 - 10985560
AN - SCOPUS:0034617468
SN - 1387-2273
VL - 744
SP - 1
EP - 8
JO - Journal of Chromatography B: Biomedical Sciences and Applications
JF - Journal of Chromatography B: Biomedical Sciences and Applications
IS - 1
ER -