HIV-1 RNA was quantitated directly by capillary electrophoresis with laser-induced fluorescence (CE-LIF) CE-LIF was used to analyze cellular RNA and various nucleotide complexes. A fluorescently labeled DNA probe (DNA/RNA complex) in conjunction with thiazole orange intercalator was determined to have optimal stability and sensitivity for RNA analysis. Based on this observation, a hybridization method using a HIV-specific fluorescently labeled probe with analysis by CE-LIF was developed. Plasma samples from a HIV-seropositive patient were lysed to obtain RNA, hybridized with the HIV-specific probe and analyzed by CE-LIF. As little as 19 fg (1710 copies per 1 ml of starting plasma) of HIV RNA can be reliably and quantitatively detected. CE-LIF appears to be an efficient and sensitive method to quantitatively analyze RNA from a variety of sources.
|Number of pages||6|
|Journal||Journal of Chromatography B: Biomedical Applications|
|State||Published - 1997|
- Gene quantification
- Human immunodeficiency virus (HIV)
ASJC Scopus subject areas
- Chemistry (all)