Direct sequencing of polymerase chain reaction (PCR) products by using the Maxam-Gilbert method is described. In this method, one of the primers is end labeled. Thus it is possible to sequence the reaction product directly following purification using this chemical method.
|Number of pages||2|
|Journal||Gene Analysis Techniques|
|State||Published - Sep 1990|
Bibliographical noteFunding Information:
This work was supported by a fellowship of the Gottlieb Daimler-und Karl Benz-Stiftung #2.88.9 (S.S.), NIMH grant MH 38819 (J. Brosius), and the American Paralysis Association and the Toyota USA Foundation (EL.). The authors wish to thank T. Kirchhausen for suggesting the approach and discussions.
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