Direct sequencing of PCR products using the Maxam-Gilbert method

Stefan Stamm, Frank M. Longo

Research output: Contribution to journalArticlepeer-review

4 Scopus citations


Direct sequencing of polymerase chain reaction (PCR) products by using the Maxam-Gilbert method is described. In this method, one of the primers is end labeled. Thus it is possible to sequence the reaction product directly following purification using this chemical method.

Original languageEnglish
Pages (from-to)142-143
Number of pages2
JournalGene Analysis Techniques
Issue number5
StatePublished - Sep 1990

Bibliographical note

Funding Information:
This work was supported by a fellowship of the Gottlieb Daimler-und Karl Benz-Stiftung #2.88.9 (S.S.), NIMH grant MH 38819 (J. Brosius), and the American Paralysis Association and the Toyota USA Foundation (EL.). The authors wish to thank T. Kirchhausen for suggesting the approach and discussions.

ASJC Scopus subject areas

  • Genetics


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