Abstract
Direct sequencing of polymerase chain reaction (PCR) products by using the Maxam-Gilbert method is described. In this method, one of the primers is end labeled. Thus it is possible to sequence the reaction product directly following purification using this chemical method.
| Original language | English |
|---|---|
| Pages (from-to) | 142-143 |
| Number of pages | 2 |
| Journal | Gene Analysis Techniques |
| Volume | 7 |
| Issue number | 5 |
| DOIs | |
| State | Published - Sep 1990 |
Bibliographical note
Funding Information:This work was supported by a fellowship of the Gottlieb Daimler-und Karl Benz-Stiftung #2.88.9 (S.S.), NIMH grant MH 38819 (J. Brosius), and the American Paralysis Association and the Toyota USA Foundation (EL.). The authors wish to thank T. Kirchhausen for suggesting the approach and discussions.
Funding
This work was supported by a fellowship of the Gottlieb Daimler-und Karl Benz-Stiftung #2.88.9 (S.S.), NIMH grant MH 38819 (J. Brosius), and the American Paralysis Association and the Toyota USA Foundation (EL.). The authors wish to thank T. Kirchhausen for suggesting the approach and discussions.
| Funders | Funder number |
|---|---|
| American Paralysis Association | |
| National Institute of Mental Health | R01MH038819 |
| Toyota Motor Manufacturing USA Inc |
ASJC Scopus subject areas
- Genetics