Disease resistance in plants that carry a feedback-regulated yeast poly(A) binding protein gene

Balasubrahmanyam Addepalli, Ruqiang Xu, Tomal Dattaroy, Baochun Li, W. Troy Bass, Qingshun Q. Li, Arthur G. Hunt

Research output: Contribution to journalArticlepeer-review

4 Scopus citations


It has been reported that the expression of the yeast poly(A) binding protein gene (PAB1) in plants leads to an induction of disease resistance responses, accompanied by alterations in the growth habit of the plant (Li et al. Plant Mol. Biol. (2000) 42 335). To capitalize on this observation, a feedback-regulated PAB1 gene was assembled and introduced into tobacco and Arabidopsis. The regulation entailed the linking of the expression of the PAB1 gene to control by the lac repressor, and by linking lac repressor expression to the disease resistance state of the plant, such that the induction of systemic defense responses by accumulation of the yeast poly(A) binding protein would turn off the expression of the PAB1 gene. Plants containing this system showed elevated and/or constitutive expression of disease-associated genes and significant resistance to otherwise pathogenic organisms. As well, they displayed a nearly normal growth habit under laboratory and greenhouse settings. These studies indicate that the expression of cytotoxic genes (such as the PAB1 gene) in plants can be controlled so that enhanced disease resistance can be achieved without significantly affecting plant growth and development.

Original languageEnglish
Pages (from-to)383-397
Number of pages15
JournalPlant Molecular Biology
Issue number3
StatePublished - Jun 2006

Bibliographical note

Funding Information:
The authors are grateful for the assistance of Carol von Lanken at various stages of this project. We also thank for Dr. Hongyan Xing’s early efforts in disease resistance tests. This study was supported by grants from the Consortium for Biotechnology Research, Inc. and the Kentucky Tobacco Research and Development Center, and by funds from Miami University.


  • Constitutive PR gene expression
  • Disease resistance
  • Feedback regulation
  • Lac repressor
  • Yeast poly(A) binding protein

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Plant Science


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