Disruption of endothelial barrier function: relationship to fluidity of membrane extracellular lamella

Angelina Alvarado; D. Allan Burrerfield; Bernhard Hennig

doi:10.1016/0020-711X(94)90016-7

Disruption of endothelial barrier function: relationship to fluidity of membrane extracellular lamella

Angelina Alvarado, D. Allan Burrerfield, Bernhard Hennig

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

1. 1. Endothelial cells were cultured in tissue culture flasks or on microcarrier beads and labeled with a lipid specific spin-label. 2. 2. Exposure of endothelial cells to benzyl alcohol caused a dose- and time-dependent increase in membrane fluidity using electron spin resonance (ESR). Maximum fluidity was reached after a 5-min exposure to 100 mM benzyl alcohol. 3. 3. Albumin permeability across endothelial cells cultured on micropore filters was used as an indication of endothelial monolayer integrity. 4. 4. A significant increase in permeability occurred with 50 mM benzyl alcohol. Maximal albumin permeability was reached after a 5-min exposure to 100 mM benzyl alcohol.

Original language	English
Pages (from-to)	575-581
Number of pages	7
Journal	International Journal of Biochemistry
Volume	26
Issue number	4
DOIs	https://doi.org/10.1016/0020-711X(94)90016-7
State	Published - Apr 1994

Bibliographical note

Funding Information:
Acknowledgemenrs-This work was supported in part by grants from the National Institutes of Health (HL36552 and AG10836), National Science Foundation (EHR9108764), National Dairy Council, National Live Stock and Meat Board, and Kentucky Agricultural Experiment Station.

Funding

Acknowledgemenrs-This work was supported in part by grants from the National Institutes of Health (HL36552 and AG10836), National Science Foundation (EHR9108764), National Dairy Council, National Live Stock and Meat Board, and Kentucky Agricultural Experiment Station.

Funders	Funder number
National Dairy Council
National Live Stock and Meat Board
National Science Foundation (NSF)	EHR9108764
National Institutes of Health (NIH)	AG10836
National Heart, Lung, and Blood Institute (NHLBI)	P01HL036552
Kentucky Agricultural Experiment Station

ASJC Scopus subject areas

Biochemistry

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10.1016/0020-711X(94)90016-7

Cite this

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title = "Disruption of endothelial barrier function: relationship to fluidity of membrane extracellular lamella",

abstract = "1. 1. Endothelial cells were cultured in tissue culture flasks or on microcarrier beads and labeled with a lipid specific spin-label. 2. 2. Exposure of endothelial cells to benzyl alcohol caused a dose- and time-dependent increase in membrane fluidity using electron spin resonance (ESR). Maximum fluidity was reached after a 5-min exposure to 100 mM benzyl alcohol. 3. 3. Albumin permeability across endothelial cells cultured on micropore filters was used as an indication of endothelial monolayer integrity. 4. 4. A significant increase in permeability occurred with 50 mM benzyl alcohol. Maximal albumin permeability was reached after a 5-min exposure to 100 mM benzyl alcohol.",

author = "Angelina Alvarado and Burrerfield, {D. Allan} and Bernhard Hennig",

note = "Funding Information: Acknowledgemenrs-This work was supported in part by grants from the National Institutes of Health (HL36552 and AG10836), National Science Foundation (EHR9108764), National Dairy Council, National Live Stock and Meat Board, and Kentucky Agricultural Experiment Station.",

year = "1994",

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doi = "10.1016/0020-711X(94)90016-7",

language = "English",

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T2 - relationship to fluidity of membrane extracellular lamella

AU - Alvarado, Angelina

AU - Burrerfield, D. Allan

AU - Hennig, Bernhard

N1 - Funding Information: Acknowledgemenrs-This work was supported in part by grants from the National Institutes of Health (HL36552 and AG10836), National Science Foundation (EHR9108764), National Dairy Council, National Live Stock and Meat Board, and Kentucky Agricultural Experiment Station.

PY - 1994/4

Y1 - 1994/4

N2 - 1. 1. Endothelial cells were cultured in tissue culture flasks or on microcarrier beads and labeled with a lipid specific spin-label. 2. 2. Exposure of endothelial cells to benzyl alcohol caused a dose- and time-dependent increase in membrane fluidity using electron spin resonance (ESR). Maximum fluidity was reached after a 5-min exposure to 100 mM benzyl alcohol. 3. 3. Albumin permeability across endothelial cells cultured on micropore filters was used as an indication of endothelial monolayer integrity. 4. 4. A significant increase in permeability occurred with 50 mM benzyl alcohol. Maximal albumin permeability was reached after a 5-min exposure to 100 mM benzyl alcohol.

AB - 1. 1. Endothelial cells were cultured in tissue culture flasks or on microcarrier beads and labeled with a lipid specific spin-label. 2. 2. Exposure of endothelial cells to benzyl alcohol caused a dose- and time-dependent increase in membrane fluidity using electron spin resonance (ESR). Maximum fluidity was reached after a 5-min exposure to 100 mM benzyl alcohol. 3. 3. Albumin permeability across endothelial cells cultured on micropore filters was used as an indication of endothelial monolayer integrity. 4. 4. A significant increase in permeability occurred with 50 mM benzyl alcohol. Maximal albumin permeability was reached after a 5-min exposure to 100 mM benzyl alcohol.

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Disruption of endothelial barrier function: relationship to fluidity of membrane extracellular lamella

Abstract

Bibliographical note

Funding

ASJC Scopus subject areas

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