1. 1. Endothelial cells were cultured in tissue culture flasks or on microcarrier beads and labeled with a lipid specific spin-label. 2. 2. Exposure of endothelial cells to benzyl alcohol caused a dose- and time-dependent increase in membrane fluidity using electron spin resonance (ESR). Maximum fluidity was reached after a 5-min exposure to 100 mM benzyl alcohol. 3. 3. Albumin permeability across endothelial cells cultured on micropore filters was used as an indication of endothelial monolayer integrity. 4. 4. A significant increase in permeability occurred with 50 mM benzyl alcohol. Maximal albumin permeability was reached after a 5-min exposure to 100 mM benzyl alcohol.
|Number of pages||7|
|Journal||International Journal of Biochemistry|
|State||Published - Apr 1994|
Bibliographical noteFunding Information:
Acknowledgemenrs-This work was supported in part by grants from the National Institutes of Health (HL36552 and AG10836), National Science Foundation (EHR9108764), National Dairy Council, National Live Stock and Meat Board, and Kentucky Agricultural Experiment Station.
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