Dissecting virus-plant interactions through proteomics approaches

Research output: Contribution to journalReview articlepeer-review

17 Scopus citations

Abstract

Plant viruses exploit cellular factors, including host proteins, membranes and metabolites, for their replication in infected cells and to establish systemic infections. Besides traditional genetic, molecular, cellular and biochemical methods for studying plant-virus interactions, both global and specialized proteomics methods are emerging as useful approaches for the identification of all the host proteins that play roles in virus infections. The various proteomics approaches include measuring differential protein expression in virus-infected versus noninfected cells, analysis of viral and host protein components in the viral replicase or other virus-induced complexes, as well as proteome-wide screens to identify host protein - viral protein interactions using protein arrays or yeast two-hybrid assays. In this review, we will discuss the progress made in plant virology using various proteomics methods, and highlight the functions of some of the identified host proteins during viral infections. Since global proteomics approaches do not usually identify the molecular mechanisms of the identified host factors during viral infections, additional experiments using genetics, biochemistry, cell biology and other approaches will often prove necessary to characterize the functions of host factors. Overall, the ever-improving proteomics approaches promise further understanding of plant-virus interactions that will likely result in new strategies for viral disease control in plants.

Original languageEnglish
Pages (from-to)316-327
Number of pages12
JournalCurrent Proteomics
Volume7
Issue number4
DOIs
StatePublished - 2010

Keywords

  • Host factors
  • Host-virus interaction
  • Plant
  • Protein microarray
  • Protein-protein interaction
  • Proteome
  • RNA binding
  • Virus
  • Virus replication

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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