Dissociation of Mouse Olfactory Mucosae for Fluorescence-Activated Cell Sorting of Olfactory Sensory Neurons

Qiang Wang; Tomoko Sengoku; William B. Titlow; Jennifer L. Strange; Timothy S. McClintock

doi:10.1007/978-1-0716-3425-7_9

Dissociation of Mouse Olfactory Mucosae for Fluorescence-Activated Cell Sorting of Olfactory Sensory Neurons

Qiang Wang, Tomoko Sengoku, William B. Titlow, Jennifer L. Strange, Timothy S. McClintock

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

Abstract

An increasing diversity of techniques investigating the biology of specific cell types and individual cells have elevated the importance of dissociation of viable cells from living tissues. Here we describe a method for the dissociation of single cells from samples of adult mouse olfactory mucosae, with an emphasis on maximizing yield of viable single cells from fluorescence-activated cell sorting. Yields are typically in the range of 80,000–150,000 viable cells per adult mouse.

Original language	English
Title of host publication	Methods in Molecular Biology
Pages	111-120
Number of pages	10
DOIs	https://doi.org/10.1007/978-1-0716-3425-7_9
State	Published - 2023

Publication series

Name	Methods in Molecular Biology
Volume	2710
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Bibliographical note

Publisher Copyright:
© The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2023.

Keywords

ChIP-seq
FACS
GFP
Olfaction
RNA-seq
Single-cell RNA-seq
Smell

ASJC Scopus subject areas

Molecular Biology
Genetics

Access to Document

10.1007/978-1-0716-3425-7_9

Cite this

@inbook{71b0f0539e454e7fb6e85be7ab2e493b,

title = "Dissociation of Mouse Olfactory Mucosae for Fluorescence-Activated Cell Sorting of Olfactory Sensory Neurons",

abstract = "An increasing diversity of techniques investigating the biology of specific cell types and individual cells have elevated the importance of dissociation of viable cells from living tissues. Here we describe a method for the dissociation of single cells from samples of adult mouse olfactory mucosae, with an emphasis on maximizing yield of viable single cells from fluorescence-activated cell sorting. Yields are typically in the range of 80,000–150,000 viable cells per adult mouse.",

keywords = "ChIP-seq, FACS, GFP, Olfaction, RNA-seq, Single-cell RNA-seq, Smell",

author = "Qiang Wang and Tomoko Sengoku and Titlow, {William B.} and Strange, {Jennifer L.} and McClintock, {Timothy S.}",

note = "Publisher Copyright: {\textcopyright} The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2023.",

year = "2023",

doi = "10.1007/978-1-0716-3425-7_9",

language = "English",

series = "Methods in Molecular Biology",

pages = "111--120",

booktitle = "Methods in Molecular Biology",

}

TY - CHAP

T1 - Dissociation of Mouse Olfactory Mucosae for Fluorescence-Activated Cell Sorting of Olfactory Sensory Neurons

AU - Wang, Qiang

AU - Sengoku, Tomoko

AU - Titlow, William B.

AU - Strange, Jennifer L.

AU - McClintock, Timothy S.

N1 - Publisher Copyright: © The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2023.

PY - 2023

Y1 - 2023

N2 - An increasing diversity of techniques investigating the biology of specific cell types and individual cells have elevated the importance of dissociation of viable cells from living tissues. Here we describe a method for the dissociation of single cells from samples of adult mouse olfactory mucosae, with an emphasis on maximizing yield of viable single cells from fluorescence-activated cell sorting. Yields are typically in the range of 80,000–150,000 viable cells per adult mouse.

AB - An increasing diversity of techniques investigating the biology of specific cell types and individual cells have elevated the importance of dissociation of viable cells from living tissues. Here we describe a method for the dissociation of single cells from samples of adult mouse olfactory mucosae, with an emphasis on maximizing yield of viable single cells from fluorescence-activated cell sorting. Yields are typically in the range of 80,000–150,000 viable cells per adult mouse.

KW - ChIP-seq

KW - FACS

KW - GFP

KW - Olfaction

KW - RNA-seq

KW - Single-cell RNA-seq

KW - Smell

UR - http://www.scopus.com/inward/record.url?scp=85170344214&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85170344214&partnerID=8YFLogxK

U2 - 10.1007/978-1-0716-3425-7_9

DO - 10.1007/978-1-0716-3425-7_9

M3 - Chapter

C2 - 37688728

AN - SCOPUS:85170344214

T3 - Methods in Molecular Biology

SP - 111

EP - 120

BT - Methods in Molecular Biology

ER -

Dissociation of Mouse Olfactory Mucosae for Fluorescence-Activated Cell Sorting of Olfactory Sensory Neurons

Abstract

Publication series

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this