Distinct transcriptomic responses of Caenorhabditis elegans to pristine and sulfidized silver nanoparticles

Daniel L. Starnes; Stuart S. Lichtenberg; Jason M. Unrine; Catherine P. Starnes; Emily K. Oostveen; Gregory V. Lowry; Paul M. Bertsch; Olga V. Tsyusko

doi:10.1016/j.envpol.2016.01.020

Distinct transcriptomic responses of Caenorhabditis elegans to pristine and sulfidized silver nanoparticles

Daniel L. Starnes
, Stuart S. Lichtenberg
, Jason M. Unrine
, Catherine P. Starnes
, Emily K. Oostveen
, Gregory V. Lowry
, Paul M. Bertsch
, Olga V. Tsyusko

Research output: Contribution to journal › Article › peer-review

46 Scopus citations

Abstract

Manufactured nanoparticles (MNP) rapidly undergo aging processes once released from products. Silver sulfide (Ag₂S) is the major transformation product formed during the wastewater treatment process for Ag-MNP. We examined toxicogenomic responses of pristine Ag-MNP, sulfidized Ag-MNP (sAg-MNP), and AgNO₃ to a model soil organism, Caenorhabditis elegans. Transcriptomic profiling of nematodes which were exposed at the EC₃₀ for reproduction for AgNO₃, Ag-MNP, and sAg-MNP resulted in 571 differentially expressed genes. We independently verified expression of 4 genes (numr-1, rol-8, col-158, and grl-20) using qRT-PCR. Only 11% of differentially expressed genes were common among the three treatments. Gene ontology enrichment analysis also revealed that Ag-MNP and sAg-MNP had distinct toxicity mechanisms and did not share any of the biological processes. The processes most affected by Ag-MNP relate to metabolism, while those processes most affected by sAg-MNP relate to molting and the cuticle, and the most impacted processes for AgNO₃ exposed nematodes was stress related. Additionally, as observed from qRT-PCR and mutant experiments, the responses to sAg-MNP were distinct from AgNO₃ while some of the effects of pristine MNP were similar to AgNO₃, suggesting that effects from Ag-MNP is partially due to dissolved silver ions.

Original language	English
Pages (from-to)	314-321
Number of pages	8
Journal	Environmental Pollution
Volume	213
DOIs	https://doi.org/10.1016/j.envpol.2016.01.020
State	Published - Jun 1 2016

Bibliographical note

Publisher Copyright:
© 2016 Published by Elsevier Ltd.

Funding

We acknowledge D. Wall and C. Chen for microarray services and R. Ma, B. Collin, D. Arndt, S. Shrestha, and A. Wamucho for laboratory assistance. Caenorhabditis elegans strains were provided by the Caenorhabditis Genetics Center, which is funded by the NIH Office of Research Infrastructure Programs ( P40 OD010440 ). Funding for this research was provided by the United States Environmental Protection Agency (U.S. EPA) Science to Achieve Results Grant RD 834574 . JU, GL and OT are supported by the U.S. EPA and National Science Foundation (NSF) under NSF cooperative agreement EF-0830093 and DBI-1266252 , Center for the Environmental Implications of NanoTechnology . SL was partial funded by the Tracy Farmer Institute for Sustainability and the Environment . Any opinions, findings, conclusions, or recommendations expressed in this material are those of the author(s) and do not necessarily reflect the views of the EPA or NSF. This work has not been subjected to EPA or NSF review, and no official endorsement should be inferred.

Funders	Funder number
National Science Foundation Arctic Social Science Program	DBI-1266252, EF-0830093
National Institutes of Health (NIH)	P40 OD010440
U.S. Environmental Protection Agency	RD 834574
Center for the Environmental Implications of NanoTechnology (CEINT)
Tracy Farmer Institute for Sustainability and the Environment, University of Kentucky

Keywords

Antimicrobial
Nanomaterial
Soil
Toxicogenomics
Wastewater

ASJC Scopus subject areas

Toxicology
Pollution
Health, Toxicology and Mutagenesis

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10.1016/j.envpol.2016.01.020

Cite this

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title = "Distinct transcriptomic responses of Caenorhabditis elegans to pristine and sulfidized silver nanoparticles",

abstract = "Manufactured nanoparticles (MNP) rapidly undergo aging processes once released from products. Silver sulfide (Ag2S) is the major transformation product formed during the wastewater treatment process for Ag-MNP. We examined toxicogenomic responses of pristine Ag-MNP, sulfidized Ag-MNP (sAg-MNP), and AgNO3 to a model soil organism, Caenorhabditis elegans. Transcriptomic profiling of nematodes which were exposed at the EC30 for reproduction for AgNO3, Ag-MNP, and sAg-MNP resulted in 571 differentially expressed genes. We independently verified expression of 4 genes (numr-1, rol-8, col-158, and grl-20) using qRT-PCR. Only 11\% of differentially expressed genes were common among the three treatments. Gene ontology enrichment analysis also revealed that Ag-MNP and sAg-MNP had distinct toxicity mechanisms and did not share any of the biological processes. The processes most affected by Ag-MNP relate to metabolism, while those processes most affected by sAg-MNP relate to molting and the cuticle, and the most impacted processes for AgNO3 exposed nematodes was stress related. Additionally, as observed from qRT-PCR and mutant experiments, the responses to sAg-MNP were distinct from AgNO3 while some of the effects of pristine MNP were similar to AgNO3, suggesting that effects from Ag-MNP is partially due to dissolved silver ions.",

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author = "Starnes, \{Daniel L.\} and Lichtenberg, \{Stuart S.\} and Unrine, \{Jason M.\} and Starnes, \{Catherine P.\} and Oostveen, \{Emily K.\} and Lowry, \{Gregory V.\} and Bertsch, \{Paul M.\} and Tsyusko, \{Olga V.\}",

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AU - Oostveen, Emily K.

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AB - Manufactured nanoparticles (MNP) rapidly undergo aging processes once released from products. Silver sulfide (Ag2S) is the major transformation product formed during the wastewater treatment process for Ag-MNP. We examined toxicogenomic responses of pristine Ag-MNP, sulfidized Ag-MNP (sAg-MNP), and AgNO3 to a model soil organism, Caenorhabditis elegans. Transcriptomic profiling of nematodes which were exposed at the EC30 for reproduction for AgNO3, Ag-MNP, and sAg-MNP resulted in 571 differentially expressed genes. We independently verified expression of 4 genes (numr-1, rol-8, col-158, and grl-20) using qRT-PCR. Only 11% of differentially expressed genes were common among the three treatments. Gene ontology enrichment analysis also revealed that Ag-MNP and sAg-MNP had distinct toxicity mechanisms and did not share any of the biological processes. The processes most affected by Ag-MNP relate to metabolism, while those processes most affected by sAg-MNP relate to molting and the cuticle, and the most impacted processes for AgNO3 exposed nematodes was stress related. Additionally, as observed from qRT-PCR and mutant experiments, the responses to sAg-MNP were distinct from AgNO3 while some of the effects of pristine MNP were similar to AgNO3, suggesting that effects from Ag-MNP is partially due to dissolved silver ions.

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KW - Nanomaterial

KW - Soil

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ER -

Distinct transcriptomic responses of Caenorhabditis elegans to pristine and sulfidized silver nanoparticles

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

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