Disulfide-linked S100β dimers and signal transduction

Steven W. Barger, Sandra R. Wolchok, Linda J. Van Eldik

Research output: Contribution to journalArticlepeer-review

112 Scopus citations


S100β is a calcium-binding protein with neurotrophic and mitogenic activities, both of which may be mediated by the protein's ability to stimulate an increase in intracellular free calcium ([Ca2+]). These extracellular trophic activities of S100β require a disulfide-linked, dimeric form of the protein. In this chapter, we present a minireview on the current state of knowledge concerning extracellular functions of S100β, with emphasis on the potential relevance of these activities to neuropathological disorders. We also report a simplified procedure for preparation of pharmacological amounts of biologically active S100β dimers, based on the finding that formation of disulfide-linked S100β dimers can be stimulated by the presence of calcium or lipid.

Original languageEnglish
Pages (from-to)105-112
Number of pages8
JournalBBA - Protein Structure
Issue number1
StatePublished - Nov 10 1992

Bibliographical note

Funding Information:
These studies were supported in part by National Institutes of Health grants NS29215 and AG10208, and Biomedical Research Support Grant RR05424. We thank Drs. Thomas Lukas, Stefan Strack and D. Martin Watterson for helpful discussions during the course of these studies and Dr. Fridolin Sulser for the use of the laser densitometer. We are also grateful to Drs. J. Oliver Mclntyre and Sidney Fleischer for the gifts of phospholipids and advice concerning their preparation and use. Finally, the help of Dr. Stefan Strack and Ms. Jessica Taylor with some of the lipid studies is gratefully acknowledged.


  • Calcium
  • Disulfide
  • Growth factor
  • Lipid
  • Neurotrophic factor
  • S100

ASJC Scopus subject areas

  • Molecular Biology
  • Structural Biology
  • Biophysics
  • Biochemistry


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