Disulfide-linked S100β dimers and signal transduction

Steven W. Barger; Sandra R. Wolchok; Linda J. Van Eldik

doi:10.1016/0167-4838(92)90043-D

Disulfide-linked S100β dimers and signal transduction

Steven W. Barger, Sandra R. Wolchok, Linda J. Van Eldik

Research output: Contribution to journal › Article › peer-review

112 Scopus citations

Abstract

S100β is a calcium-binding protein with neurotrophic and mitogenic activities, both of which may be mediated by the protein's ability to stimulate an increase in intracellular free calcium ([Ca²⁺]). These extracellular trophic activities of S100β require a disulfide-linked, dimeric form of the protein. In this chapter, we present a minireview on the current state of knowledge concerning extracellular functions of S100β, with emphasis on the potential relevance of these activities to neuropathological disorders. We also report a simplified procedure for preparation of pharmacological amounts of biologically active S100β dimers, based on the finding that formation of disulfide-linked S100β dimers can be stimulated by the presence of calcium or lipid.

Original language	English
Pages (from-to)	105-112
Number of pages	8
Journal	BBA - Protein Structure
Volume	1160
Issue number	1
DOIs	https://doi.org/10.1016/0167-4838(92)90043-D
State	Published - Nov 10 1992

Bibliographical note

Funding Information:
These studies were supported in part by National Institutes of Health grants NS29215 and AG10208, and Biomedical Research Support Grant RR05424. We thank Drs. Thomas Lukas, Stefan Strack and D. Martin Watterson for helpful discussions during the course of these studies and Dr. Fridolin Sulser for the use of the laser densitometer. We are also grateful to Drs. J. Oliver Mclntyre and Sidney Fleischer for the gifts of phospholipids and advice concerning their preparation and use. Finally, the help of Dr. Stefan Strack and Ms. Jessica Taylor with some of the lipid studies is gratefully acknowledged.

Keywords

Calcium
Disulfide
Growth factor
Lipid
Neurotrophic factor
S100

ASJC Scopus subject areas

Molecular Biology
Structural Biology
Biophysics
Biochemistry

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10.1016/0167-4838(92)90043-D

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title = "Disulfide-linked S100β dimers and signal transduction",

abstract = "S100β is a calcium-binding protein with neurotrophic and mitogenic activities, both of which may be mediated by the protein's ability to stimulate an increase in intracellular free calcium ([Ca2+]). These extracellular trophic activities of S100β require a disulfide-linked, dimeric form of the protein. In this chapter, we present a minireview on the current state of knowledge concerning extracellular functions of S100β, with emphasis on the potential relevance of these activities to neuropathological disorders. We also report a simplified procedure for preparation of pharmacological amounts of biologically active S100β dimers, based on the finding that formation of disulfide-linked S100β dimers can be stimulated by the presence of calcium or lipid.",

keywords = "Calcium, Disulfide, Growth factor, Lipid, Neurotrophic factor, S100",

author = "Barger, {Steven W.} and Wolchok, {Sandra R.} and {Van Eldik}, {Linda J.}",

note = "Funding Information: These studies were supported in part by National Institutes of Health grants NS29215 and AG10208, and Biomedical Research Support Grant RR05424. We thank Drs. Thomas Lukas, Stefan Strack and D. Martin Watterson for helpful discussions during the course of these studies and Dr. Fridolin Sulser for the use of the laser densitometer. We are also grateful to Drs. J. Oliver Mclntyre and Sidney Fleischer for the gifts of phospholipids and advice concerning their preparation and use. Finally, the help of Dr. Stefan Strack and Ms. Jessica Taylor with some of the lipid studies is gratefully acknowledged.",

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doi = "10.1016/0167-4838(92)90043-D",

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AU - Barger, Steven W.

AU - Wolchok, Sandra R.

AU - Van Eldik, Linda J.

N1 - Funding Information: These studies were supported in part by National Institutes of Health grants NS29215 and AG10208, and Biomedical Research Support Grant RR05424. We thank Drs. Thomas Lukas, Stefan Strack and D. Martin Watterson for helpful discussions during the course of these studies and Dr. Fridolin Sulser for the use of the laser densitometer. We are also grateful to Drs. J. Oliver Mclntyre and Sidney Fleischer for the gifts of phospholipids and advice concerning their preparation and use. Finally, the help of Dr. Stefan Strack and Ms. Jessica Taylor with some of the lipid studies is gratefully acknowledged.

PY - 1992/11/10

Y1 - 1992/11/10

N2 - S100β is a calcium-binding protein with neurotrophic and mitogenic activities, both of which may be mediated by the protein's ability to stimulate an increase in intracellular free calcium ([Ca2+]). These extracellular trophic activities of S100β require a disulfide-linked, dimeric form of the protein. In this chapter, we present a minireview on the current state of knowledge concerning extracellular functions of S100β, with emphasis on the potential relevance of these activities to neuropathological disorders. We also report a simplified procedure for preparation of pharmacological amounts of biologically active S100β dimers, based on the finding that formation of disulfide-linked S100β dimers can be stimulated by the presence of calcium or lipid.

AB - S100β is a calcium-binding protein with neurotrophic and mitogenic activities, both of which may be mediated by the protein's ability to stimulate an increase in intracellular free calcium ([Ca2+]). These extracellular trophic activities of S100β require a disulfide-linked, dimeric form of the protein. In this chapter, we present a minireview on the current state of knowledge concerning extracellular functions of S100β, with emphasis on the potential relevance of these activities to neuropathological disorders. We also report a simplified procedure for preparation of pharmacological amounts of biologically active S100β dimers, based on the finding that formation of disulfide-linked S100β dimers can be stimulated by the presence of calcium or lipid.

KW - Calcium

KW - Disulfide

KW - Growth factor

KW - Lipid

KW - Neurotrophic factor

KW - S100

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Disulfide-linked S100β dimers and signal transduction

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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