Diverse polyketides from the marine endophytic Alternaria sp. LV52: Structure determination and cytotoxic activities

Manar M. Mahmoud, Ahmed S. Abdel-Razek, Hesham S.M. Soliman, Larissa V. Ponomareva, Jon S. Thorson, Khaled A. Shaaban, Mohamed Shaaban

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

We report the isolation and characterization of five polyketides [alternariol (1), alternariol-9-methyl ether (2), altertoxin I (3), altertoxin II (4) and tenuazonic acid (5)] from the marine endophytic Alternaria sp. LV52 derived from Cystoseira tamariscifolia, collected from the Red Sea at Nabq-Bay, Egypt. The chemical structures of compounds 1–5 were identified by extensive 1D, 2D NMR, and HR mass measurements. Isolation and phenotypic and genotypic characterization of the producing fungus is reported. The antimicrobial activity of the produced extract and derived compounds was examined against a panel of test organisms. In addition, an in vitro cytotoxic activity of 1–5 was performed against diverse cancer cell lines: HEPG2, HELA, A549 and PC3, revealing that compounds 2 and 4 are potentially cytotoxic against A549 and PC3 with EC50 of 0.73 µg/ml (2.69 µM) and 0.17 µg/ml (0.64 µM) for 2, and 0.40 µg/ml (1.15 µM) and 0.12 µg/ml (0.33 µM) for 4, respectively.

Original languageEnglish
Article numbere00628
JournalBiotechnology Reports
Volume33
DOIs
StatePublished - Mar 2022

Bibliographical note

Publisher Copyright:
© 2021 The Authors

Keywords

  • Alternaria sp.
  • Cytotoxicity
  • Marine endophyte
  • Polyketides
  • Taxonomy

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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