Diverse population of a new bipartite begomovirus infecting tomato crops in Uruguay

Belén Márquez-Martín, Diego Maeso, Ada Martínez-Ayala, Roberto Bernal, M. Teresa Federici, Paul Vincelli, Jesús Navas-Castillo, Enrique Moriones

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10 Scopus citations


Several isolates of a novel begomovirus were characterized from tomato samples collected in northern Uruguay exhibiting disease symptoms associated with Bemisia tabaci infestations. Analysis of full-length sequences of DNA-A and DNA-B components revealed the presence of a new begomovirus with the typical genome organization of a New World begomovirus, for which the name tomato rugose yellow leaf curl virus (ToRYLCV) is proposed. A high degree of nucleotide sequence diversity was found for both components, suggesting the presence of a diverse virus population. Recombination analysis suggested relationships of ToRYLCV to begomoviruses reported from the New World. Although common regions from DNA-As and DNA-Bs were surprisingly divergent for a cognate pair, a DNA-A and DNA-B pair cloned from one sample were infectious in Nicotiana benthamiana and tomato and reproduced symptoms observed in field-infected tomato plants, suggesting that ToRYLCV is the causal agent of the disease observed. This is the first report of a begomovirus infecting tomato crops in Uruguay and of the presence of begomovirus in this country.

Original languageEnglish
Pages (from-to)1137-1142
Number of pages6
JournalArchives of Virology
Issue number6
StatePublished - Jun 2012

Bibliographical note

Funding Information:
This work was supported by grant GEN2006-27770-C2-2 (Ministerio de Educación y Ciencia, Spain, co-financed by FEDER) in the frame of the ERA-NET Plant Genomics project ERA-PG 040B RCA-Genomics (6th EU Framework Programme for Research and Technological Development, FP6), and Network 111RT0433 from Fundación CYTED. J. N.-C. and E. M. are members of the Research Group AGR-214, partially funded by the Consejería de Economía, Innovación y Ciencia, Junta de Andalucía, Spain (cofinanced by FEDER-FSE).

ASJC Scopus subject areas

  • Virology


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