Diverse population of a new bipartite begomovirus infecting tomato crops in Uruguay

Belén Márquez-Martín; Diego Maeso; Ada Martínez-Ayala; Roberto Bernal; M. Teresa Federici; Paul Vincelli; Jesús Navas-Castillo; Enrique Moriones

doi:10.1007/s00705-012-1262-6

Diverse population of a new bipartite begomovirus infecting tomato crops in Uruguay

Belén Márquez-Martín, Diego Maeso, Ada Martínez-Ayala, Roberto Bernal, M. Teresa Federici, Paul Vincelli, Jesús Navas-Castillo, Enrique Moriones

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Several isolates of a novel begomovirus were characterized from tomato samples collected in northern Uruguay exhibiting disease symptoms associated with Bemisia tabaci infestations. Analysis of full-length sequences of DNA-A and DNA-B components revealed the presence of a new begomovirus with the typical genome organization of a New World begomovirus, for which the name tomato rugose yellow leaf curl virus (ToRYLCV) is proposed. A high degree of nucleotide sequence diversity was found for both components, suggesting the presence of a diverse virus population. Recombination analysis suggested relationships of ToRYLCV to begomoviruses reported from the New World. Although common regions from DNA-As and DNA-Bs were surprisingly divergent for a cognate pair, a DNA-A and DNA-B pair cloned from one sample were infectious in Nicotiana benthamiana and tomato and reproduced symptoms observed in field-infected tomato plants, suggesting that ToRYLCV is the causal agent of the disease observed. This is the first report of a begomovirus infecting tomato crops in Uruguay and of the presence of begomovirus in this country.

Original language	English
Pages (from-to)	1137-1142
Number of pages	6
Journal	Archives of Virology
Volume	157
Issue number	6
DOIs	https://doi.org/10.1007/s00705-012-1262-6
State	Published - Jun 2012

Bibliographical note

Funding Information:
This work was supported by grant GEN2006-27770-C2-2 (Ministerio de Educación y Ciencia, Spain, co-financed by FEDER) in the frame of the ERA-NET Plant Genomics project ERA-PG 040B RCA-Genomics (6th EU Framework Programme for Research and Technological Development, FP6), and Network 111RT0433 from Fundación CYTED. J. N.-C. and E. M. are members of the Research Group AGR-214, partially funded by the Consejería de Economía, Innovación y Ciencia, Junta de Andalucía, Spain (cofinanced by FEDER-FSE).

Funding

This work was supported by grant GEN2006-27770-C2-2 (Ministerio de Educación y Ciencia, Spain, co-financed by FEDER) in the frame of the ERA-NET Plant Genomics project ERA-PG 040B RCA-Genomics (6th EU Framework Programme for Research and Technological Development, FP6), and Network 111RT0433 from Fundación CYTED. J. N.-C. and E. M. are members of the Research Group AGR-214, partially funded by the Consejería de Economía, Innovación y Ciencia, Junta de Andalucía, Spain (cofinanced by FEDER-FSE).

Funders	Funder number
6th EU Framework Programme for Research and Technological Development
FEDER-FSE
Fundación CYTED	AGR-214
Ministerio de Educación y Ciencia, Spain
Sixth Framework Programme	111RT0433
Consejería de Economía, Innovación, Ciencia y Empleo, Junta de Andalucía
European Regional Development Fund

ASJC Scopus subject areas

Virology

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10.1007/s00705-012-1262-6

Cite this

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title = "Diverse population of a new bipartite begomovirus infecting tomato crops in Uruguay",

abstract = "Several isolates of a novel begomovirus were characterized from tomato samples collected in northern Uruguay exhibiting disease symptoms associated with Bemisia tabaci infestations. Analysis of full-length sequences of DNA-A and DNA-B components revealed the presence of a new begomovirus with the typical genome organization of a New World begomovirus, for which the name tomato rugose yellow leaf curl virus (ToRYLCV) is proposed. A high degree of nucleotide sequence diversity was found for both components, suggesting the presence of a diverse virus population. Recombination analysis suggested relationships of ToRYLCV to begomoviruses reported from the New World. Although common regions from DNA-As and DNA-Bs were surprisingly divergent for a cognate pair, a DNA-A and DNA-B pair cloned from one sample were infectious in Nicotiana benthamiana and tomato and reproduced symptoms observed in field-infected tomato plants, suggesting that ToRYLCV is the causal agent of the disease observed. This is the first report of a begomovirus infecting tomato crops in Uruguay and of the presence of begomovirus in this country.",

author = "Bel{\'e}n M{\'a}rquez-Mart{\'i}n and Diego Maeso and Ada Mart{\'i}nez-Ayala and Roberto Bernal and Federici, \{M. Teresa\} and Paul Vincelli and Jes{\'u}s Navas-Castillo and Enrique Moriones",

note = "Funding Information: This work was supported by grant GEN2006-27770-C2-2 (Ministerio de Educaci{\'o}n y Ciencia, Spain, co-financed by FEDER) in the frame of the ERA-NET Plant Genomics project ERA-PG 040B RCA-Genomics (6th EU Framework Programme for Research and Technological Development, FP6), and Network 111RT0433 from Fundaci{\'o}n CYTED. J. N.-C. and E. M. are members of the Research Group AGR-214, partially funded by the Consejer{\'i}a de Econom{\'i}a, Innovaci{\'o}n y Ciencia, Junta de Andaluc{\'i}a, Spain (cofinanced by FEDER-FSE).",

year = "2012",

month = jun,

doi = "10.1007/s00705-012-1262-6",

language = "English",

volume = "157",

pages = "1137--1142",

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AU - Maeso, Diego

AU - Martínez-Ayala, Ada

AU - Bernal, Roberto

AU - Federici, M. Teresa

AU - Vincelli, Paul

AU - Navas-Castillo, Jesús

AU - Moriones, Enrique

N1 - Funding Information: This work was supported by grant GEN2006-27770-C2-2 (Ministerio de Educación y Ciencia, Spain, co-financed by FEDER) in the frame of the ERA-NET Plant Genomics project ERA-PG 040B RCA-Genomics (6th EU Framework Programme for Research and Technological Development, FP6), and Network 111RT0433 from Fundación CYTED. J. N.-C. and E. M. are members of the Research Group AGR-214, partially funded by the Consejería de Economía, Innovación y Ciencia, Junta de Andalucía, Spain (cofinanced by FEDER-FSE).

PY - 2012/6

Y1 - 2012/6

N2 - Several isolates of a novel begomovirus were characterized from tomato samples collected in northern Uruguay exhibiting disease symptoms associated with Bemisia tabaci infestations. Analysis of full-length sequences of DNA-A and DNA-B components revealed the presence of a new begomovirus with the typical genome organization of a New World begomovirus, for which the name tomato rugose yellow leaf curl virus (ToRYLCV) is proposed. A high degree of nucleotide sequence diversity was found for both components, suggesting the presence of a diverse virus population. Recombination analysis suggested relationships of ToRYLCV to begomoviruses reported from the New World. Although common regions from DNA-As and DNA-Bs were surprisingly divergent for a cognate pair, a DNA-A and DNA-B pair cloned from one sample were infectious in Nicotiana benthamiana and tomato and reproduced symptoms observed in field-infected tomato plants, suggesting that ToRYLCV is the causal agent of the disease observed. This is the first report of a begomovirus infecting tomato crops in Uruguay and of the presence of begomovirus in this country.

AB - Several isolates of a novel begomovirus were characterized from tomato samples collected in northern Uruguay exhibiting disease symptoms associated with Bemisia tabaci infestations. Analysis of full-length sequences of DNA-A and DNA-B components revealed the presence of a new begomovirus with the typical genome organization of a New World begomovirus, for which the name tomato rugose yellow leaf curl virus (ToRYLCV) is proposed. A high degree of nucleotide sequence diversity was found for both components, suggesting the presence of a diverse virus population. Recombination analysis suggested relationships of ToRYLCV to begomoviruses reported from the New World. Although common regions from DNA-As and DNA-Bs were surprisingly divergent for a cognate pair, a DNA-A and DNA-B pair cloned from one sample were infectious in Nicotiana benthamiana and tomato and reproduced symptoms observed in field-infected tomato plants, suggesting that ToRYLCV is the causal agent of the disease observed. This is the first report of a begomovirus infecting tomato crops in Uruguay and of the presence of begomovirus in this country.

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Diverse population of a new bipartite begomovirus infecting tomato crops in Uruguay

Abstract

Bibliographical note

Funding

ASJC Scopus subject areas

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