Division-Independent Differentiation of Muscle Stem Cells During a Growth Stimulus

Ahmed Ismaeel, Jensen Goh, C. Brooks Mobley, Kevin A. Murach, Jamie O. Brett, Antoine De MorrCrossed D Sign©e, Thomas A. Rando, Charlotte A. Peterson, Yuan Wen, John J. McCarthy

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

dult muscle stem cells (MuSCs) are known to replicate upon activation before differentiating and fusing to regenerate myofibers. It is unclear whether MuSC differentiation is intrinsically linked to cell division, which has implications for stem cell population maintenance. We use single-cell RNA-sequencing to identify transcriptionally diverse subpopulations of MuSCs after 5 days of a growth stimulus in adult muscle. Trajectory inference in combination with a novel mouse model for tracking MuSC-derived myonuclei and in vivo labeling of DNA replication revealed an MuSC population that exhibited division-independent differentiation and fusion. These findings demonstrate that in response to a growth stimulus in the presence of intact myofibers, MuSC division is not obligatory.

Original languageEnglish
Pages (from-to)266-277
Number of pages12
JournalStem Cells
Volume42
Issue number3
DOIs
StatePublished - Mar 2024

Bibliographical note

Publisher Copyright:
© 2023 The Author(s). Published by Oxford University Press.

Keywords

  • DNA replication
  • adult stem cells
  • cell division
  • single-cell gene expression analysis

ASJC Scopus subject areas

  • General Medicine

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