Division-Independent Differentiation of Muscle Stem Cells During a Growth Stimulus

Ahmed Ismaeel; Jensen Goh; C. Brooks Mobley; Kevin A. Murach; Jamie O. Brett; Antoine De MorrCrossed D Sign©e; Thomas A. Rando; Charlotte A. Peterson; Yuan Wen; John J. McCarthy

doi:10.1093/stmcls/sxad091

Division-Independent Differentiation of Muscle Stem Cells During a Growth Stimulus

Ahmed Ismaeel, Jensen Goh, C. Brooks Mobley, Kevin A. Murach, Jamie O. Brett, Antoine De MorrCrossed D Sign©e, Thomas A. Rando, Charlotte A. Peterson, Yuan Wen, John J. McCarthy

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

dult muscle stem cells (MuSCs) are known to replicate upon activation before differentiating and fusing to regenerate myofibers. It is unclear whether MuSC differentiation is intrinsically linked to cell division, which has implications for stem cell population maintenance. We use single-cell RNA-sequencing to identify transcriptionally diverse subpopulations of MuSCs after 5 days of a growth stimulus in adult muscle. Trajectory inference in combination with a novel mouse model for tracking MuSC-derived myonuclei and in vivo labeling of DNA replication revealed an MuSC population that exhibited division-independent differentiation and fusion. These findings demonstrate that in response to a growth stimulus in the presence of intact myofibers, MuSC division is not obligatory.

Original language	English
Pages (from-to)	266-277
Number of pages	12
Journal	Stem Cells
Volume	42
Issue number	3
DOIs	https://doi.org/10.1093/stmcls/sxad091
State	Published - Mar 2024

Bibliographical note

Publisher Copyright:
© 2023 The Author(s). Published by Oxford University Press.

Keywords

DNA replication
adult stem cells
cell division
single-cell gene expression analysis

ASJC Scopus subject areas

General Medicine

Access to Document

10.1093/stmcls/sxad091

Cite this

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title = "Division-Independent Differentiation of Muscle Stem Cells During a Growth Stimulus",

abstract = "dult muscle stem cells (MuSCs) are known to replicate upon activation before differentiating and fusing to regenerate myofibers. It is unclear whether MuSC differentiation is intrinsically linked to cell division, which has implications for stem cell population maintenance. We use single-cell RNA-sequencing to identify transcriptionally diverse subpopulations of MuSCs after 5 days of a growth stimulus in adult muscle. Trajectory inference in combination with a novel mouse model for tracking MuSC-derived myonuclei and in vivo labeling of DNA replication revealed an MuSC population that exhibited division-independent differentiation and fusion. These findings demonstrate that in response to a growth stimulus in the presence of intact myofibers, MuSC division is not obligatory.",

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doi = "10.1093/stmcls/sxad091",

language = "English",

volume = "42",

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AU - Ismaeel, Ahmed

AU - Goh, Jensen

AU - Mobley, C. Brooks

AU - Murach, Kevin A.

AU - Brett, Jamie O.

AU - De MorrCrossed D Sign©e, Antoine

AU - Rando, Thomas A.

AU - Peterson, Charlotte A.

AU - Wen, Yuan

AU - McCarthy, John J.

PY - 2024/3

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AB - dult muscle stem cells (MuSCs) are known to replicate upon activation before differentiating and fusing to regenerate myofibers. It is unclear whether MuSC differentiation is intrinsically linked to cell division, which has implications for stem cell population maintenance. We use single-cell RNA-sequencing to identify transcriptionally diverse subpopulations of MuSCs after 5 days of a growth stimulus in adult muscle. Trajectory inference in combination with a novel mouse model for tracking MuSC-derived myonuclei and in vivo labeling of DNA replication revealed an MuSC population that exhibited division-independent differentiation and fusion. These findings demonstrate that in response to a growth stimulus in the presence of intact myofibers, MuSC division is not obligatory.

KW - DNA replication

KW - adult stem cells

KW - cell division

KW - single-cell gene expression analysis

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Division-Independent Differentiation of Muscle Stem Cells During a Growth Stimulus

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

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