DNA-AgNC Loaded Liposomes for Measuring Cerebral Blood Flow Using Two-Photon Fluorescence Correlation Spectroscopy

Xiaojin Wang, Mikkel B. Liisberg, Georgia L. Nolt, Xu Fu, Cecilia Cerretani, Lan Li, Lance A. Johnson, Tom Vosch, Christopher I. Richards

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Unraveling the transport of drugs and nanocarriers in cerebrovascular networks is important for pharmacokinetic and hemodynamic studies but is challenging due to the complexity of sensing individual particles within the circulatory system of a live animal. Here, we demonstrate that a DNA-stabilized silver nanocluster (DNA-Ag16NC) that emits in the first near-infrared window upon two-photon excitation in the second NIR window can be used for multiphoton in vivo fluorescence correlation spectroscopy for the measurement of cerebral blood flow rates in live mice with high spatial and temporal resolution. To ensure bright and stable emission during in vivo experiments, we loaded DNA-Ag16NCs into liposomes, which served the dual purposes of concentrating the fluorescent label and protecting it from degradation. DNA-Ag16NC-loaded liposomes enabled the quantification of cerebral blood flow velocities within individual vessels of a living mouse.

Original languageEnglish
Pages (from-to)12862-12874
Number of pages13
JournalACS Nano
Volume17
Issue number13
DOIs
StatePublished - Jul 11 2023

Bibliographical note

Publisher Copyright:
© 2023 American Chemical Society.

Keywords

  • DNA-AgNCs
  • FCS
  • cerebral blood flow (CBF)
  • in vivo
  • liposomes

ASJC Scopus subject areas

  • General Materials Science
  • General Engineering
  • General Physics and Astronomy

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