TY - JOUR
T1 - DNA-binding properties of cosmomycin D, an anthracycline with two trisaccharide chains
AU - Furlan, Renata L.A.
AU - Watt, Stephen J.
AU - Garrido, Leandro M.
AU - Amarante-Mendes, Gustavo P.
AU - Nur-E-Alam, Mohammad
AU - Rohr, Jurgen
AU - Braña, Alfredo
AU - Mendez, Carmen
AU - Salas, Jose A.
AU - Sheil, Margaret M.
AU - Beck, Jennifer L.
AU - Padilla, Gabriel
PY - 2004/10
Y1 - 2004/10
N2 - Cosmomycin D (CosD) is the major constituent fraction isolated from a culture of Streptomyces olindensis ICB20. The ability of this compound to intercalate with double-stranded DNA was studied by gel mobility shift assays and electrospray ionization mass spectrometry (ESI-MS). ESI-MS experiments showed that the complex of CosD with 16-mer double-stranded DNA was at least as stable as a complex of daunorubicin with the same DNA sequence. This is the first study showing DNA binding properties of an anthracycline containing a β-rhodomycinone aglycone chromophore O-linked to two trisaccharide chains.
AB - Cosmomycin D (CosD) is the major constituent fraction isolated from a culture of Streptomyces olindensis ICB20. The ability of this compound to intercalate with double-stranded DNA was studied by gel mobility shift assays and electrospray ionization mass spectrometry (ESI-MS). ESI-MS experiments showed that the complex of CosD with 16-mer double-stranded DNA was at least as stable as a complex of daunorubicin with the same DNA sequence. This is the first study showing DNA binding properties of an anthracycline containing a β-rhodomycinone aglycone chromophore O-linked to two trisaccharide chains.
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U2 - 10.7164/antibiotics.57.647
DO - 10.7164/antibiotics.57.647
M3 - Article
C2 - 15638325
AN - SCOPUS:8144220812
SN - 0021-8820
VL - 57
SP - 647
EP - 654
JO - Journal of Antibiotics
JF - Journal of Antibiotics
IS - 10
ER -