DNA damage associated with PCBs in the whole blood cells of Inuit

Srivani Ravoori, Pierre Ayotte, Cidambi Srinivasan, Daria Pereg, Larry W. Robertson, Gilandra K. Russell, Jeyaprakash Jeyabalan, Ramesh C. Gupta

Research output: Contribution to journalArticlepeer-review

11 Scopus citations


Lower chlorinated PCBs can damage DNA directly or via free radical mechanisms. In order to assess the DNA-damaging potential of PCBs in humans, blood samples were collected from Inuit population from Salluit, Northern Canada. Their diet comprises blubber from sea mammals and fatty fish, which accumulate non-biodegradable PCBs at varying levels. The 103 samples thus collected were categorized into low-, medium- and high-PCB exposure groups. A comprehensive 32P-postlabeling adductomics technology, which allows measure differences in DNA adduct profiles of polar and lipophilic adducts between control and exposure groups, was applied to these samples to assess the effect of PCB on DNA damage. The adduct patterns obtained were qualitatively similar to other human tissues studied previously. A range of highly polar to lipophilic subgroups of adducts were detected. The known oxidative lesion, 8-oxodG was predominant. While some individual adducts appear to accumulate with increasing PCB levels, a definitive association could not be made. A possible confounder effect of selenium is discussed.

Original languageEnglish
Pages (from-to)273-276
Number of pages4
JournalEnvironmental Toxicology and Pharmacology
Issue number2
StatePublished - Mar 2008

Bibliographical note

Funding Information:
This work was supported from ES 07380 and in part from the Agnes Brown Duggan Endowment Funds.

Copyright 2008 Elsevier B.V., All rights reserved.


  • DNA adducts
  • Inuit
  • P-postlabeling
  • PCBs

ASJC Scopus subject areas

  • Toxicology
  • Pharmacology
  • Health, Toxicology and Mutagenesis


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