Donnan potential across the outer membrane of gram-negative bacteria and its effect on the permeability of antibiotics

Olaniyi Alegun; Ankit Pandeya; Jian Cui; Isoiza Ojo; Yinan Wei

doi:10.3390/antibiotics10060701

Donnan potential across the outer membrane of gram-negative bacteria and its effect on the permeability of antibiotics

Olaniyi Alegun, Ankit Pandeya, Jian Cui, Isoiza Ojo, Yinan Wei

Chemistry

Research output: Contribution to journal › Review article › peer-review

30 Scopus citations

Abstract

The cell envelope structure of Gram-negative bacteria is unique, composed of two lipid bilayer membranes and an aqueous periplasmic space sandwiched in between. The outer membrane constitutes an extra barrier to limit the exchange of molecules between the cells and the exterior environment. Donnan potential is a membrane potential across the outer membrane, resulted from the selective permeability of the membrane, which plays a pivotal role in the permeability of many antibiotics. In this review, we discussed factors that affect the intensity of the Donnan potential, including the osmotic strength and pH of the external media, the osmoregulated periplasmic glucans trapped in the periplasmic space, and the displacement of cell surface charges. The focus of our discussion is the impact of Donnan potential on the cellular permeability of selected antibiotics including fluoroquinolones, tetracyclines, β-lactams, and trimethoprim.

Original language	English
Article number	701
Journal	Antibiotics
Volume	10
Issue number	6
DOIs	https://doi.org/10.3390/antibiotics10060701
State	Published - Jun 2021

Bibliographical note

Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.

Funding

This research was funded by NSF CHE-1709381, NIH/NIAID AI137020 and AI142063, NIH/NHLBI HL142640, and NIH/NIGMS GM132443.

Funders	Funder number
National Institutes of Health (NIH)
Division of Microbiology and Infectious Diseases, National Institute of Allergy and Infectious Diseases	AI142063, AI137020
U.S. Department of Energy Chinese Academy of Sciences Guangzhou Municipal Science and Technology Project Oak Ridge National Laboratory Extreme Science and Engineering Discovery Environment National Science Foundation National Energy Research Scientific Computing Center National Natural Science Foundation of China	CHE-1709381
National Heart, Lung, and Blood Institute Family Blood Pressure Program	HL142640
National Institute of General Medical Sciences	GM132443

Keywords

Antibiotics
Cellular accumulation
Donnan potential
Gram-negative bacteria
Membrane permeability

ASJC Scopus subject areas

Microbiology
Biochemistry
General Pharmacology, Toxicology and Pharmaceutics
Microbiology (medical)
Infectious Diseases
Pharmacology (medical)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.3390/antibiotics10060701

Cite this

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title = "Donnan potential across the outer membrane of gram-negative bacteria and its effect on the permeability of antibiotics",

abstract = "The cell envelope structure of Gram-negative bacteria is unique, composed of two lipid bilayer membranes and an aqueous periplasmic space sandwiched in between. The outer membrane constitutes an extra barrier to limit the exchange of molecules between the cells and the exterior environment. Donnan potential is a membrane potential across the outer membrane, resulted from the selective permeability of the membrane, which plays a pivotal role in the permeability of many antibiotics. In this review, we discussed factors that affect the intensity of the Donnan potential, including the osmotic strength and pH of the external media, the osmoregulated periplasmic glucans trapped in the periplasmic space, and the displacement of cell surface charges. The focus of our discussion is the impact of Donnan potential on the cellular permeability of selected antibiotics including fluoroquinolones, tetracyclines, β-lactams, and trimethoprim.",

keywords = "Antibiotics, Cellular accumulation, Donnan potential, Gram-negative bacteria, Membrane permeability",

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T1 - Donnan potential across the outer membrane of gram-negative bacteria and its effect on the permeability of antibiotics

AU - Alegun, Olaniyi

AU - Pandeya, Ankit

AU - Cui, Jian

AU - Ojo, Isoiza

AU - Wei, Yinan

PY - 2021/6

Y1 - 2021/6

N2 - The cell envelope structure of Gram-negative bacteria is unique, composed of two lipid bilayer membranes and an aqueous periplasmic space sandwiched in between. The outer membrane constitutes an extra barrier to limit the exchange of molecules between the cells and the exterior environment. Donnan potential is a membrane potential across the outer membrane, resulted from the selective permeability of the membrane, which plays a pivotal role in the permeability of many antibiotics. In this review, we discussed factors that affect the intensity of the Donnan potential, including the osmotic strength and pH of the external media, the osmoregulated periplasmic glucans trapped in the periplasmic space, and the displacement of cell surface charges. The focus of our discussion is the impact of Donnan potential on the cellular permeability of selected antibiotics including fluoroquinolones, tetracyclines, β-lactams, and trimethoprim.

AB - The cell envelope structure of Gram-negative bacteria is unique, composed of two lipid bilayer membranes and an aqueous periplasmic space sandwiched in between. The outer membrane constitutes an extra barrier to limit the exchange of molecules between the cells and the exterior environment. Donnan potential is a membrane potential across the outer membrane, resulted from the selective permeability of the membrane, which plays a pivotal role in the permeability of many antibiotics. In this review, we discussed factors that affect the intensity of the Donnan potential, including the osmotic strength and pH of the external media, the osmoregulated periplasmic glucans trapped in the periplasmic space, and the displacement of cell surface charges. The focus of our discussion is the impact of Donnan potential on the cellular permeability of selected antibiotics including fluoroquinolones, tetracyclines, β-lactams, and trimethoprim.

KW - Antibiotics

KW - Cellular accumulation

KW - Donnan potential

KW - Gram-negative bacteria

KW - Membrane permeability

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UR - https://www.scopus.com/inward/citedby.url?scp=85108705385&partnerID=8YFLogxK

U2 - 10.3390/antibiotics10060701

DO - 10.3390/antibiotics10060701

M3 - Review article

AN - SCOPUS:85108705385

VL - 10

JO - Antibiotics

JF - Antibiotics

IS - 6

M1 - 701

ER -

Donnan potential across the outer membrane of gram-negative bacteria and its effect on the permeability of antibiotics

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

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