Dopamine D2-like receptor binding in the brain of male Japanese quail (Coturnix japonica)

Neil Levens, Thomas A. Green, Chana K. Akins, Michael T. Bardo

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

Japanese quail (Coturnix japonica) have been used extensively to study appetitive behaviors. However, little is known about the appetitive-relevant neurochemical systems in this species. The present investigation examined the distribution of D2-like dopamine receptors in the quail brain. [3H]Spiperone was incubated in brain tissue homogenates and non-specific binding was defined using (-)-sulpiride. Scatchard analysis of whole brain without cerebellum and forebrain alone indicated approximate K(d)'s of 0.08 and 0.04 nM, respectively. In addition, the preferential D3 agonist (±)-2-dipropylamino-7-hydroxy-1,2,3,4-tetrahydronaphthalene hydrobromide (7-OH-DPAT) did not displace [3H]spiperone binding in quail forebrain. Finally, regional analysis showed that the highest densities of D2-like receptors were located in the forebrain. Overall, these results indicate that there is some conservation of dopaminergic mechanisms between aves and mammals. Thus, Japanese quail may be useful for examining the neuropharmacological mechanisms of dopaminergic stimulant drugs that work via D2-like receptor activation. (C) 2000 Elsevier Science Ireland Ltd.

Original languageEnglish
Pages (from-to)77-80
Number of pages4
JournalNeuroscience Letters
Volume296
Issue number2-3
DOIs
StatePublished - Dec 22 2000

Bibliographical note

Funding Information:
The authors wish to think Scott Phillips for his help in the laboratory. M.T.B. and C.K.A. supported by USPHS grant DA05312 and National Science Foundation IBN9728756, respectively. N.L. and T.A.G. supported by the Research Challenge Trust Fund and the Interdepartmental Neuroscience Program at the University of Kentucky.

Keywords

  • Avian species
  • Birds
  • D-like receptor
  • Dopamine
  • Quail
  • Reward
  • [H]Spiperone binding

ASJC Scopus subject areas

  • General Neuroscience

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