Dopamine Stimulates [3H]Phorbol 12,13‐Dibutyrate Binding in Cultured Striatal Cells

M. K. McMillian, X. P. He, J. S. Hong, K. R. Pennypacker

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

Abstract: The effect of dopamine (DA) on the binding of [3H]phorbol 12,13‐dibutyrate ([3H]PdBu) in cultured rat striatal cells was examined. DA maximally increased specific [3H]PdBu binding by 70 ± 10%, an increase comparable to that observed with norepinephrine (NE). This finding suggests that DA activates protein kinase C in cultured striatal cells, because increases in [3H]PdBu binding reflect translocation of protein kinase C. Half‐maximal stimulation was observed with 10–6M DA. The peak response was observed at 2–3 min after addition of 10–4M DA, but [3H]PdBu binding was still increased above basal at 30 min. DA was not acting via an adrenergic receptor. Prazosin (10–6M) blocked the response to NE, suggesting mediation by an α1‐adrenergic receptor, but had little effect on the response to DA. Conversely, the D1 receptor antagonist SCH‐23390 (10–6M) blocked the response to DA, but only partially inhibited the response to NE. Morphine (10–6M) inhibited the response to DA by 46 ± 14%, but did not affect significantly the response to NE. The DA effect on [3H]PdBu binding is apparently independent of the increase in cyclic AMP seen on D1 receptor activation. Forskolin, apomorphine, and the D1 agonist SKF‐38393 all increased cyclic AMP in striatal cells, but were less effective than DA in stimulating [3H]PdBu binding. The D2 agonist quinpirole was ineffective in stimulating either cyclic AMP or [3H]PdBu binding.

Original languageEnglish
Pages (from-to)1308-1312
Number of pages5
JournalJournal of Neurochemistry
Volume58
Issue number4
DOIs
StatePublished - Apr 1992

Keywords

  • Dopamine
  • Norepinephrine
  • Rat
  • Striatal cells
  • [H]Phorbol 12,13‐dibutyrate

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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