Downregulation of microRNA eca-mir-128 in seminal exosomes and enhanced expression of CXCL16 in the stallion reproductive tract are associated with long-term persistence of equine arteritis virus

Mariano Carossino, Pouya Dini, Theodore S. Kalbfleisch, Alan T. Loynachan, Igor F. Canisso, Kathleen M. Shuck, Peter J. Timoney, R. Frank Cook, Udeni B.R. Balasuriya

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

Equine arteritis virus (EAV) can establish long-term persistent infection in the reproductive tract of stallions and is shed in the semen. Previous studies showed that long-term persistence is associated with a specific allele of the CXCL16 gene (CXCL16S) and that persistent infection is maintained despite the presence of a local inflammatory and humoral and mucosal antibody responses. In this study, we demonstrated that equine seminal exosomes (SEs) are enriched in a small subset of microRNAs (miRNAs). Most importantly, we demonstrated that long-term EAV persistence is associated with the downregulation of an SE-associated miRNA (eca-mir- 128) and with an enhanced expression of CXCL16 in the reproductive tract, a putative target of eca-mir-128. The findings presented here suggest that SE eca-mir-128 is implicated in the regulation of the CXCL16/CXCR6 axis in the reproductive tract of persistently infected stallions, a chemokine axis strongly implicated in EAV persistence. This is a novel finding and warrants further investigation to identify its specific mechanism in modulating the CXCL16/CXCR6 axis in the reproductive tract of the EAV long-term carrier stallion.

Original languageEnglish
Article numbere00015-18
JournalJournal of Virology
Volume92
Issue number9
DOIs
StatePublished - May 1 2018

Bibliographical note

Publisher Copyright:
© 2018 American Society for Microbiology.

Keywords

  • CXCL16
  • EAV
  • EVA
  • Eca-mir-128
  • Equine arteritis virus
  • Equine viral arteritis
  • MiRNA
  • Persistent infection
  • Reproductive tract
  • Seminal exosomes

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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