Background: Ventral hernia is a commonly occurring surgical problem. Our earlier studies have shown that a 30 mg/kg dose of doxycycline can significantly impact the strength of polypropylene (PP) mesh in a rat hernia repair model at 6 and 12 weeks. The objective of the present study was to investigate the dose dependence of doxycycline treatment on hernia repair strengths in rats. Study design: Fifty-six Sprague-Dawley rats underwent hernia repair with either PP mesh (n = 28) or sutures only (primary; n = 28); both groups were further divided into four doxycycline groups of seven animals each: control (0 mg/kg), low (3 mg/kg), medium (10 mg/kg), and high (30 mg/kg). One day before hernia repair surgery, animals received doxycycline doses by gavage and continued receiving daily until euthanasia. After 8 weeks, rats were euthanized and tissue samples from hernia repaired area were collected and analyzed for tensile strength using a tensiometer (Instron, Canton, MA, USA), while MMPs 2, 3, and 9, and collagen type 1 and 3 were analyzed by western blotting. Results: In mesh-repaired animals, medium and high doxycycline dose repaired mesh fascia interface (MFI) showed significant increase in tensile strength when compared to control. In the primary repaired animals, there was no significant difference in MFI tensile strength in any dose group. In medium-dose MFI, there was a significant reduction in MMPs 2, 3, and 9. In this animal group, MFI showed significant increase in collagen 1 and significant reduction in collagen type 3 when compared to control. Conclusion: It is possible to improve the strength of mesh-repaired tissue by administering a significantly lower dose of the drug, which has implications for translation of the findings.
|Number of pages||6|
|State||Published - May 1 2016|
Bibliographical noteFunding Information:
This research was supported by a grant from Davol, Inc., Warwick, Rhode Island. Timely help in the preparation and submission of the manuscript by Ms. Linda Combs is greatly appreciated. Drs. Tharappel, Harris, Zwischenberger, and Levy have no conflicts of interest or financial ties to disclose. Dr. Puleo receives Grant funding from the NIH (R01AR060964). Dr. Roth has received external grant funding from Davol, Inc. for the support of this study; he has also received external grant funding and consulting fees from CR Bard; he is on speaker bureaus for CR Bard and Ethicon; and he is a Board member of the Musculoskeletal Transplant Foundation.
© 2015, Springer Science+Business Media New York.
- Extracellular matrix
- Hernia repair
- Matrix metalloproteinase
ASJC Scopus subject areas