DREADDs in drosophila: A pharmacogenetic approach for controlling behavior, neuronal signaling, and physiology in the fly

Jaime Becnel, Oralee Johnson, Zana R. Majeed, Vi Tran, Bangning Yu, Bryan L. Roth, Robin L. Cooper, Edmund K. Kerut, Charles D. Nichols

Research output: Contribution to journalArticlepeer-review

39 Scopus citations

Abstract

We have translated a powerful genetic tool, designer receptors exclusively activated by designer drugs (DREADDs), from mammalian systems to Drosophila melanogaster to selectively, rapidly, reversibly, and dose-dependently control behaviors and physiological processes in the fly. DREADDs are muscarinic acetylcholine G protein-coupled receptors evolved for loss of affinity to acetylcholine and for the ability to be fully activated by an otherwise biologically inert chemical, clozapine-N-oxide. We demonstrate its ability to control a variety of behaviors and processes in larvae and adults, including heart rate, sensory processing, diurnal behavior, learning and memory, and courtship. The advantages of this particular technology include the dose-responsive control of behaviors, the lack of a need for specialized equipment, and the capacity to remotely control signaling in essentially all neuronal and nonneuronal fly tissues

Original languageEnglish
Pages (from-to)1049-1059
Number of pages11
JournalCell Reports
Volume4
Issue number5
DOIs
StatePublished - May 12 2013

Bibliographical note

Funding Information:
We would like to thank Dr. Dan Mangiavellano for comments on the manuscript and the Higher Committee for Education Development in Iraq (Z.R.M.). This work was funded by R01MH083689 (C.D.N).

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology (all)

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