We have translated a powerful genetic tool, designer receptors exclusively activated by designer drugs (DREADDs), from mammalian systems to Drosophila melanogaster to selectively, rapidly, reversibly, and dose-dependently control behaviors and physiological processes in the fly. DREADDs are muscarinic acetylcholine G protein-coupled receptors evolved for loss of affinity to acetylcholine and for the ability to be fully activated by an otherwise biologically inert chemical, clozapine-N-oxide. We demonstrate its ability to control a variety of behaviors and processes in larvae and adults, including heart rate, sensory processing, diurnal behavior, learning and memory, and courtship. The advantages of this particular technology include the dose-responsive control of behaviors, the lack of a need for specialized equipment, and the capacity to remotely control signaling in essentially all neuronal and nonneuronal fly tissues
|Number of pages||11|
|State||Published - May 12 2013|
Bibliographical noteFunding Information:
We would like to thank Dr. Dan Mangiavellano for comments on the manuscript and the Higher Committee for Education Development in Iraq (Z.R.M.). This work was funded by R01MH083689 (C.D.N).
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology (all)