RNA interference (RNAi) is inefficient in lepidopteran insects, including Spodoptera frugiperda. RNase activity in the lumen and hemocoel is known to contribute to low RNAi efficiency in these insects. Conditioned medium from Sf9 cells developed from ovaries of S. frugiperda shows high dsRNA degradation activity. But the enzymes responsible for this activity have not been identified. The nuclease genes that are highly expressed in Sf9 cells, REase, RNaseT2, and dsRNase1, were identified. Knockdown of dsRNase1 in Sf9 cells resulted in a reduction of dsRNA degradation activity in the Sf9 cells conditioned medium. Knockdown of dsRNase1 also increased RNAi efficiency in Sf9 cells. The results from these studies identified a major player in dsRNA degradation activity in the Sf9 cells conditioned medium. We also describe an efficient system that can be used to identify other genes responsible for dsRNA degradation and RNAi efficiency in Sf9 cells.
|Journal||Frontiers in Insect Science|
|State||Published - 2023|
Bibliographical noteFunding Information:
This Research reported in this thesis was supported by the National Institute of General Medical Sciences and National Institute of National Institute of Allergy and Infectious diseases of the National Institutes of Health under Award Numbers R01GM070559 and R21AI163561-02. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.
Copyright © 2023 Koo and Palli.
- dsRNA stability
- fall armyworm
- Spodoptera frugiperda
ASJC Scopus subject areas
- Insect Science