Dynamic assembly of surface structures in living cells

Julia Gorelik; Andrew I. Shevchuk; Gregory I. Frolenkov; Ivan A. Diakonov; Max J. Lab; Corné J. Kros; Guy P. Richardson; Igor Vodyanoy; Christopher R.W. Edwards; David Klenerman; Yuri E. Korchev

doi:10.1073/pnas.1030502100

Dynamic assembly of surface structures in living cells

Julia Gorelik, Andrew I. Shevchuk, Gregory I. Frolenkov, Ivan A. Diakonov, Max J. Lab, Corné J. Kros, Guy P. Richardson, Igor Vodyanoy, Christopher R.W. Edwards, David Klenerman, Yuri E. Korchev

Physiology

Research output: Contribution to journal › Article › peer-review

145 Scopus citations

Abstract

Although the dynamics of cell membranes and associated structures is vital for cell function, little is known due to lack of suitable methods. We found, using scanning ion conductance microscopy, that microvilli, membrane projections supported by internal actin bundles, undergo a life cycle: fast height-dependent growth, relatively short steady state, and slow height-independent retraction. The microvilli can aggregate into relatively stable structures where the steady state is extended. We suggest that the intrinsic dynamics of microvilli, combined with their ability to make stable structures, allows them to act as elementary "building blocks" for the assembly of specialized structures on the cell surface.

Original language	English
Pages (from-to)	5819-5822
Number of pages	4
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	100
Issue number	10
DOIs	https://doi.org/10.1073/pnas.1030502100
State	Published - May 13 2003

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Gorelik, J., Shevchuk, A. I., Frolenkov, G. I., Diakonov, I. A., Lab, M. J., Kros, C. J., Richardson, G. P., Vodyanoy, I., Edwards, C. R. W., Klenerman, D., & Korchev, Y. E. (2003). Dynamic assembly of surface structures in living cells. Proceedings of the National Academy of Sciences of the United States of America, 100(10), 5819-5822. https://doi.org/10.1073/pnas.1030502100

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doi = "10.1073/pnas.1030502100",

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AU - Gorelik, Julia

AU - Shevchuk, Andrew I.

AU - Frolenkov, Gregory I.

AU - Diakonov, Ivan A.

AU - Lab, Max J.

AU - Kros, Corné J.

AU - Richardson, Guy P.

AU - Vodyanoy, Igor

AU - Edwards, Christopher R.W.

AU - Klenerman, David

AU - Korchev, Yuri E.

PY - 2003/5/13

Y1 - 2003/5/13

N2 - Although the dynamics of cell membranes and associated structures is vital for cell function, little is known due to lack of suitable methods. We found, using scanning ion conductance microscopy, that microvilli, membrane projections supported by internal actin bundles, undergo a life cycle: fast height-dependent growth, relatively short steady state, and slow height-independent retraction. The microvilli can aggregate into relatively stable structures where the steady state is extended. We suggest that the intrinsic dynamics of microvilli, combined with their ability to make stable structures, allows them to act as elementary "building blocks" for the assembly of specialized structures on the cell surface.

AB - Although the dynamics of cell membranes and associated structures is vital for cell function, little is known due to lack of suitable methods. We found, using scanning ion conductance microscopy, that microvilli, membrane projections supported by internal actin bundles, undergo a life cycle: fast height-dependent growth, relatively short steady state, and slow height-independent retraction. The microvilli can aggregate into relatively stable structures where the steady state is extended. We suggest that the intrinsic dynamics of microvilli, combined with their ability to make stable structures, allows them to act as elementary "building blocks" for the assembly of specialized structures on the cell surface.

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Dynamic assembly of surface structures in living cells

Abstract

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