Dynamic assembly of surface structures in living cells

Julia Gorelik, Andrew I. Shevchuk, Gregory I. Frolenkov, Ivan A. Diakonov, Max J. Lab, Corné J. Kros, Guy P. Richardson, Igor Vodyanoy, Christopher R.W. Edwards, David Klenerman, Yuri E. Korchev

Research output: Contribution to journalArticlepeer-review

142 Scopus citations

Abstract

Although the dynamics of cell membranes and associated structures is vital for cell function, little is known due to lack of suitable methods. We found, using scanning ion conductance microscopy, that microvilli, membrane projections supported by internal actin bundles, undergo a life cycle: fast height-dependent growth, relatively short steady state, and slow height-independent retraction. The microvilli can aggregate into relatively stable structures where the steady state is extended. We suggest that the intrinsic dynamics of microvilli, combined with their ability to make stable structures, allows them to act as elementary "building blocks" for the assembly of specialized structures on the cell surface.

Original languageEnglish
Pages (from-to)5819-5822
Number of pages4
JournalProceedings of the National Academy of Sciences of the United States of America
Volume100
Issue number10
DOIs
StatePublished - May 13 2003

ASJC Scopus subject areas

  • General

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