Dysregulation of genes and microRNAs in localized aggressive periodontitis

Jussara Gonçalves Fernandes, Lorri Ann Morford, Peter Lloyd Harrison, Theodora Kompotiati, Hong Huang, Ikramuddin Aukhil, Shannon Margaret Wallet, Luciana Macchion Shaddox

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

Aim: Previous data from our laboratory have demonstrated that localized aggressive periodontitis (LAP) patients produce elevated levels of pro-inflammatory cytokines in response to TLR4 and TLR2 ligation compared to unrelated and periodontally healthy controls (HC). The aim of the present work is to evaluate the contribution of TLR-related gene expression and miRNA regulation in LAP disease. Material and methods: Peripheral blood mononuclear cells (PBMCs) from LAP and health control (HC) patients were isolated. Gene and miRNA expression involved in TLR signalling pathway and immunopathology were evaluated in unstimulated PBMCs by real-time PCR (RT-PCR). Results: TICAM-1 (TRIF), FOS, IRAK1, TLR2 and CCL2 genes and the miRNAs miR-9-5p, miR-155-5p and 203a-3p, miR-147a, miR-182-5p and miR-183-5p were significantly up-regulated in LAP compared to HC. Conclusions: Most of the genes and miRNAs overexpressed here are directly or indirectly related to immune response and inflammation. This profile supports our previous findings that suggests LAP patients have a “hyper-responsive” phenotype upon activation of TLR pathway by periodontal pathogens.

Original languageEnglish
Pages (from-to)1317-1325
Number of pages9
JournalJournal of Clinical Periodontology
Volume47
Issue number11
DOIs
StatePublished - Nov 1 2020

Bibliographical note

Publisher Copyright:
© 2020 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd

ASJC Scopus subject areas

  • Periodontics

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