TY - JOUR
T1 - Dysregulation of genes and microRNAs in localized aggressive periodontitis
AU - Gonçalves Fernandes, Jussara
AU - Morford, Lorri Ann
AU - Harrison, Peter Lloyd
AU - Kompotiati, Theodora
AU - Huang, Hong
AU - Aukhil, Ikramuddin
AU - Wallet, Shannon Margaret
AU - Macchion Shaddox, Luciana
N1 - Publisher Copyright:
© 2020 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd
PY - 2020/11/1
Y1 - 2020/11/1
N2 - Aim: Previous data from our laboratory have demonstrated that localized aggressive periodontitis (LAP) patients produce elevated levels of pro-inflammatory cytokines in response to TLR4 and TLR2 ligation compared to unrelated and periodontally healthy controls (HC). The aim of the present work is to evaluate the contribution of TLR-related gene expression and miRNA regulation in LAP disease. Material and methods: Peripheral blood mononuclear cells (PBMCs) from LAP and health control (HC) patients were isolated. Gene and miRNA expression involved in TLR signalling pathway and immunopathology were evaluated in unstimulated PBMCs by real-time PCR (RT-PCR). Results: TICAM-1 (TRIF), FOS, IRAK1, TLR2 and CCL2 genes and the miRNAs miR-9-5p, miR-155-5p and 203a-3p, miR-147a, miR-182-5p and miR-183-5p were significantly up-regulated in LAP compared to HC. Conclusions: Most of the genes and miRNAs overexpressed here are directly or indirectly related to immune response and inflammation. This profile supports our previous findings that suggests LAP patients have a “hyper-responsive” phenotype upon activation of TLR pathway by periodontal pathogens.
AB - Aim: Previous data from our laboratory have demonstrated that localized aggressive periodontitis (LAP) patients produce elevated levels of pro-inflammatory cytokines in response to TLR4 and TLR2 ligation compared to unrelated and periodontally healthy controls (HC). The aim of the present work is to evaluate the contribution of TLR-related gene expression and miRNA regulation in LAP disease. Material and methods: Peripheral blood mononuclear cells (PBMCs) from LAP and health control (HC) patients were isolated. Gene and miRNA expression involved in TLR signalling pathway and immunopathology were evaluated in unstimulated PBMCs by real-time PCR (RT-PCR). Results: TICAM-1 (TRIF), FOS, IRAK1, TLR2 and CCL2 genes and the miRNAs miR-9-5p, miR-155-5p and 203a-3p, miR-147a, miR-182-5p and miR-183-5p were significantly up-regulated in LAP compared to HC. Conclusions: Most of the genes and miRNAs overexpressed here are directly or indirectly related to immune response and inflammation. This profile supports our previous findings that suggests LAP patients have a “hyper-responsive” phenotype upon activation of TLR pathway by periodontal pathogens.
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U2 - 10.1111/jcpe.13361
DO - 10.1111/jcpe.13361
M3 - Article
C2 - 32876337
AN - SCOPUS:85092141122
SN - 0303-6979
VL - 47
SP - 1317
EP - 1325
JO - Journal of Clinical Periodontology
JF - Journal of Clinical Periodontology
IS - 11
ER -