Early events in B lymphocyte activation.

Subbarao Bondada, Amy Troyer, Ralph L. Chelvarajan

Research output: Contribution to journalArticlepeer-review

1 Scopus citations


B cell activation can be quantitated indirectly by assaying antibody production or directly by measuring cellular changes that occur immediately after exposure to an activation signal. This unit provides methods for the latter (direct) approach--namely, methods for quantifying early parameters of B cell activation such as increases in intracellular ionized calcium concentration [Ca2+]I, cell size, and MHC class II-antigen expression. These assays are not so much B cell specific as activation specific; as such they are a useful means of providing information about cellular events that are independent of antibody secretion. While the protocols included in this unit have been specifically designed for use with murine B cells, they can also be used with B cells obtained from other species. In addition, the method described here also applies to T cells.

Original languageEnglish
Pages (from-to)Unit 3.9
JournalCurrent protocols in immunology / edited by John E. Coligan ... [et al.]
VolumeChapter 3
StatePublished - Nov 2003

ASJC Scopus subject areas

  • Immunology


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