Early postnatal development of calmodulin-dependent protein kinase II in rat brain

Naji Sahyoun, Harry LeVine, Susan K. Burgess, Steven Blanchard, Kwen Jen Chang, Pedro Cuatrecasas

Research output: Contribution to journalArticlepeer-review

29 Scopus citations

Abstract

The postnatal levels of the α- (Mr 50,000) and β- (Mr 58,000/60,000) subunits of type II calmodulin-dependent protein kinase were investigated in cytosolic, cytoskeletal and nuclear subfractions of rat brain. Substantial amounts of the β-subunit were present neonatally, especially in the cytoplasm; both subunits increased markedly during the subsequent three weeks with α-preponderance in the cortex and midbrain. The development of the α-subunit was attenuated in the cerebellum and brain stem, resulting in a relative excess of the β-subunit. Primary neuronal cultures from fetal brain initially displayed low enzyme content, followed by an increase in the levels of the β- but not α-subunit. Regulation of the cellular content of type II calmodulin-dependent protein kinase may be relevant to neuronal differentiation.

Original languageEnglish
Pages (from-to)878-884
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume132
Issue number3
DOIs
StatePublished - Nov 15 1985

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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