Ecdysteroid regulation of ovarian growth and oocyte maturation in the red flour beetle, Tribolium castaneum

R. Parthasarathy; Zhentao Sheng; Zhiyuan Sun; Subba R. Palli

doi:10.1016/j.ibmb.2010.04.002

Ecdysteroid regulation of ovarian growth and oocyte maturation in the red flour beetle, Tribolium castaneum

R. Parthasarathy, Zhentao Sheng, Zhiyuan Sun, Subba R. Palli

Research output: Contribution to journal › Article › peer-review

118 Scopus citations

Abstract

Previous studies from our laboratory showed the involvement of juvenile hormone (JH) and ecdysteroid signaling in the regulation of female reproduction in the red flour beetle, Tribolium castaneum. JH regulates vitellogenin (Vg) synthesis in the fat body but the role of ecdysteroid signaling is not known. Here, we report on ecdysteroid regulation of ovarian growth and oocyte maturation. Microarray analysis of RNA isolated from ovaries showed the up-regulation of several genes coding for proteins involved in ecdysteroid signaling on the 4th day after female adult eclosion. The functional analyses of genes coding for proteins involved in ecdysteroid and JH signaling pathways by RNA interference (RNAi) revealed that ecdysteroids but not JH regulate ovarian growth and primary oocyte maturation. Ultrastructural studies showed the temporal sequences of key events in oogenesis including the development of primary oocytes, the differentiation and development of follicle epithelial cells, and the formation of intercellular spaces to facilitate uptake of Vg protein. RNAi studies showed that ecdysone receptor (EcR) and ultraspiracle (USP) are required for the ovarian growth, primary oocyte maturation and the growth and migration of the follicle cells. These studies suggest important roles for ecdysteroids in the regulation of oocyte maturation in the beetle ovaries.

Original language	English
Pages (from-to)	429-439
Number of pages	11
Journal	Insect Biochemistry and Molecular Biology
Volume	40
Issue number	6
DOIs	https://doi.org/10.1016/j.ibmb.2010.04.002
State	Published - Jun 2010

Bibliographical note

Funding Information:
This work was supported by National Institutes of Health (GM070559-06). We thank Dr. Nigel Cooper and Ms. Xiahong Li of University of Louisville for help with microarray experiment. The University of Louisville microarray facility is supported by NCRR IDeA Awards INBRE-P20 RR016481 and COBRE-P20RR018733. We also thank Dr. Perry and Dr. Michael Goodin of University of Kentucky for microscope facilities. We acknowledge the assistance in electron microscopy work rendered by Mr. Jim Begley and Ms. Mary Gail Engle of the Imaging Facility, University of Kentucky. This is contribution number 10-08-058 from the Kentucky Agricultural Experimental Station.

Keywords

20E
Follicle cell
JH
Ovary
RNAi
Tribolium
Vitellogenesis

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Insect Science

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10.1016/j.ibmb.2010.04.002

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@article{174bb3222a2a46cdbd55ee03f41e4899,

title = "Ecdysteroid regulation of ovarian growth and oocyte maturation in the red flour beetle, Tribolium castaneum",

abstract = "Previous studies from our laboratory showed the involvement of juvenile hormone (JH) and ecdysteroid signaling in the regulation of female reproduction in the red flour beetle, Tribolium castaneum. JH regulates vitellogenin (Vg) synthesis in the fat body but the role of ecdysteroid signaling is not known. Here, we report on ecdysteroid regulation of ovarian growth and oocyte maturation. Microarray analysis of RNA isolated from ovaries showed the up-regulation of several genes coding for proteins involved in ecdysteroid signaling on the 4th day after female adult eclosion. The functional analyses of genes coding for proteins involved in ecdysteroid and JH signaling pathways by RNA interference (RNAi) revealed that ecdysteroids but not JH regulate ovarian growth and primary oocyte maturation. Ultrastructural studies showed the temporal sequences of key events in oogenesis including the development of primary oocytes, the differentiation and development of follicle epithelial cells, and the formation of intercellular spaces to facilitate uptake of Vg protein. RNAi studies showed that ecdysone receptor (EcR) and ultraspiracle (USP) are required for the ovarian growth, primary oocyte maturation and the growth and migration of the follicle cells. These studies suggest important roles for ecdysteroids in the regulation of oocyte maturation in the beetle ovaries.",

keywords = "20E, Follicle cell, JH, Ovary, RNAi, Tribolium, Vitellogenesis",

author = "R. Parthasarathy and Zhentao Sheng and Zhiyuan Sun and Palli, {Subba R.}",

note = "Funding Information: This work was supported by National Institutes of Health (GM070559-06). We thank Dr. Nigel Cooper and Ms. Xiahong Li of University of Louisville for help with microarray experiment. The University of Louisville microarray facility is supported by NCRR IDeA Awards INBRE-P20 RR016481 and COBRE-P20RR018733. We also thank Dr. Perry and Dr. Michael Goodin of University of Kentucky for microscope facilities. We acknowledge the assistance in electron microscopy work rendered by Mr. Jim Begley and Ms. Mary Gail Engle of the Imaging Facility, University of Kentucky. This is contribution number 10-08-058 from the Kentucky Agricultural Experimental Station.",

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AU - Palli, Subba R.

N1 - Funding Information: This work was supported by National Institutes of Health (GM070559-06). We thank Dr. Nigel Cooper and Ms. Xiahong Li of University of Louisville for help with microarray experiment. The University of Louisville microarray facility is supported by NCRR IDeA Awards INBRE-P20 RR016481 and COBRE-P20RR018733. We also thank Dr. Perry and Dr. Michael Goodin of University of Kentucky for microscope facilities. We acknowledge the assistance in electron microscopy work rendered by Mr. Jim Begley and Ms. Mary Gail Engle of the Imaging Facility, University of Kentucky. This is contribution number 10-08-058 from the Kentucky Agricultural Experimental Station.

PY - 2010/6

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N2 - Previous studies from our laboratory showed the involvement of juvenile hormone (JH) and ecdysteroid signaling in the regulation of female reproduction in the red flour beetle, Tribolium castaneum. JH regulates vitellogenin (Vg) synthesis in the fat body but the role of ecdysteroid signaling is not known. Here, we report on ecdysteroid regulation of ovarian growth and oocyte maturation. Microarray analysis of RNA isolated from ovaries showed the up-regulation of several genes coding for proteins involved in ecdysteroid signaling on the 4th day after female adult eclosion. The functional analyses of genes coding for proteins involved in ecdysteroid and JH signaling pathways by RNA interference (RNAi) revealed that ecdysteroids but not JH regulate ovarian growth and primary oocyte maturation. Ultrastructural studies showed the temporal sequences of key events in oogenesis including the development of primary oocytes, the differentiation and development of follicle epithelial cells, and the formation of intercellular spaces to facilitate uptake of Vg protein. RNAi studies showed that ecdysone receptor (EcR) and ultraspiracle (USP) are required for the ovarian growth, primary oocyte maturation and the growth and migration of the follicle cells. These studies suggest important roles for ecdysteroids in the regulation of oocyte maturation in the beetle ovaries.

AB - Previous studies from our laboratory showed the involvement of juvenile hormone (JH) and ecdysteroid signaling in the regulation of female reproduction in the red flour beetle, Tribolium castaneum. JH regulates vitellogenin (Vg) synthesis in the fat body but the role of ecdysteroid signaling is not known. Here, we report on ecdysteroid regulation of ovarian growth and oocyte maturation. Microarray analysis of RNA isolated from ovaries showed the up-regulation of several genes coding for proteins involved in ecdysteroid signaling on the 4th day after female adult eclosion. The functional analyses of genes coding for proteins involved in ecdysteroid and JH signaling pathways by RNA interference (RNAi) revealed that ecdysteroids but not JH regulate ovarian growth and primary oocyte maturation. Ultrastructural studies showed the temporal sequences of key events in oogenesis including the development of primary oocytes, the differentiation and development of follicle epithelial cells, and the formation of intercellular spaces to facilitate uptake of Vg protein. RNAi studies showed that ecdysone receptor (EcR) and ultraspiracle (USP) are required for the ovarian growth, primary oocyte maturation and the growth and migration of the follicle cells. These studies suggest important roles for ecdysteroids in the regulation of oocyte maturation in the beetle ovaries.

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JO - Insect Biochemistry and Molecular Biology

JF - Insect Biochemistry and Molecular Biology

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Ecdysteroid regulation of ovarian growth and oocyte maturation in the red flour beetle, Tribolium castaneum

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

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