Ectopic expression of ecdysone oxidase impairs tissue degeneration in Bombyx mori

Zhiqian Li, Lang You, Baosheng Zeng, Lin Ling, Jun Xu, Xu Chen, Zhongjie Zhang, Subba Reddy Palli, Yongping Huang, Anjiang Tan

Research output: Contribution to journalArticlepeer-review

43 Scopus citations

Abstract

Metamorphosis in insects includes a series of programmed tissue histolysis and remolding processesthat are controlled by two majorclasses of hormones, juvenile hormones and ecdysteroids. Precise pulses of ecdysteroids (the most active ecdysteroid is 20-hydroxyecdysone, 20E), are regulated by both bio- synthesis and metabolism. In this study, we show that ecdysone oxidase (EO), a 20E inactivation enzyme, expresses predominantly in the midgut during the early pupal stage in the lepidopteran model insect, Bombyx mori. Depletion of BmEO using the transgenic CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/RNA-guided Cas9 nucleases) system extended the duration of the final instar larval stage. Ubiquitous transgenic overexpression of BmEO using the Gal4/UAS system induced lethality during the larval-pupal transition. When BmEO was specifically over- expressed in the middle silk gland (MSG), degeneration of MSG at the onset of metamorphosis was blocked. Transmission electron microscope and LysoTrackeranalyses showed that the autophagy pathway in MSG is inhibited by BmEO ectopic expression. Furthermore, RNA-seq analysis revealed that the genes involved in autophagic cell death and the mTOR signal pathway are affected by overexpression of BmEO. Taken together, BmEO functional studies reported here provide insights into ecdysone regulation of tissue degeneration during metamorphosis.

Original languageEnglish
Article number20150513
JournalProceedings of the Royal Society B: Biological Sciences
Volume282
Issue number1809
DOIs
StatePublished - Jun 3 2015

Bibliographical note

Publisher Copyright:
© 2015 The Author(s) Published by the Royal Society. All rights reserved.

Keywords

  • Autophagy
  • Bombyx mori
  • CRISPR/Cas9
  • Ecdysone oxidase
  • Gal4/UAS

ASJC Scopus subject areas

  • General Biochemistry, Genetics and Molecular Biology
  • General Immunology and Microbiology
  • General Environmental Science
  • General Agricultural and Biological Sciences

Fingerprint

Dive into the research topics of 'Ectopic expression of ecdysone oxidase impairs tissue degeneration in Bombyx mori'. Together they form a unique fingerprint.

Cite this