Effect of α-tocopherol and tocopherol succinate on lipid peroxidation in equine spermatozoa

Juliana Almeida, Barry A. Ball

Research output: Contribution to journalArticlepeer-review

72 Scopus citations

Abstract

The objective of this study was to compare the effect of α-tocopherol and its ester, α tocopherol succinate, on lipid peroxidation and motility of equine spermatozoa. In experiment one, spermatozoa were incubated with dl-α-tocopherol (5, 25, 100 or 500 μM), dl-α tocopherol succinate (5, 25, 100 or 500 μM) or vehicle (0.5% ethanol) at 38°C, and sperm motility was determined at 30, 60 and 120 min. In experiment two, spermatozoa loaded with the lipophilic probe, C11BODIPY581/591, were incubated with dl-α-tocopherol (50 and 100 μM), dl-α-tocopherol succinate (50 and 100 μM) or ethanol (0.5%) and with the promoters cumene hydroperoxide, Fe2SO4, and ascorbate at 38°C in 5% CO2. Lipid peroxidation was determined by changes in fluorescence of C11BODIPY581/591, and motility was determined by CASA at 0, 15, 30 and 60 min. In experiment three, spermatozoa loaded with C 11BODIPY581/591 were incubated with dl-α-tocopherol (5, 25, 100 or 500 μM), dl-α-tocopherol succinate (5, 25, 100 or 500 μM) or ethanol (0.5%) at 38°C and then submitted to a 4-hour incubation at room temperature. Motility and lipid peroxidation were determined at 1 and 4 h. In experiment four, the effect of DL α tocopherol (5, 25 or 500 μM), dl-α-tocopherol succinate (5, 25 or 500 μM) or ethanol (0.5%) on lipid peroxidation and motility were evaluated during storage at 5°C in a skim-milk based extender. Although dl-α-tocopherol succinate appeared more effective than dl-α-tocopherol in preventing lipid peroxidation during short-term incubations, the succinate ester suppressed sperm motility compared to dl-α-tocopherol alone.

Original languageEnglish
Pages (from-to)321-337
Number of pages17
JournalAnimal Reproduction Science
Volume87
Issue number3-4
DOIs
StatePublished - Jul 2005

Bibliographical note

Funding Information:
This research was supported by the John P. Hughes Endowment, by the Center for Equine Health with funds provided by the Oak Tree Racing Association, the State of California pari-mutuel fund, and contributions by private donors and by the National Research Initiative Competitive Grant no. 2002-35203-12260 from the USDA Cooperative State Research, Education, and Extension Service.

Keywords

  • Equine
  • Lipid peroxidation
  • Spermatozoa
  • Tocopherol

ASJC Scopus subject areas

  • Food Animals
  • Animal Science and Zoology
  • Endocrinology

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