TY - JOUR
T1 - Effect of alloxan diabetes and subsequent insulin treatment on temperature kinetics properties of succinate oxidase activity in rat kidney mitochondria
AU - Patel, Samir P.
AU - Katyare, Surendra S.
PY - 2006/9
Y1 - 2006/9
N2 - Early and late effects of alloxan diabetes and subsequent treatment with insulin on the temperature kinetics properties of succinate oxidase (SO) activity in rat kidney mitochondria were examined. In diabetic animals SO activity increased significantly and the increase was more pronounced at the late stage. Insulin treatment partially restored SO activity. However, the effect was temperature-dependent. In diabetic animals the energy of activation in the low temperature range (EL) increased significantly while that in the high temperature range (EH) decreased. The latter seems to be responsible for improving catalytic efficiency in the diabetic state. Insulin treatment normalized EH only in the 1-month diabetic group. The phase transition temperature (Tt), decreased in diabetic animals. Insulin treatment caused an increase beyond the control value in Tt in 1-month diabetic animals. The results suggest that insulin status-dependent modulation of SO activity is a complex process.
AB - Early and late effects of alloxan diabetes and subsequent treatment with insulin on the temperature kinetics properties of succinate oxidase (SO) activity in rat kidney mitochondria were examined. In diabetic animals SO activity increased significantly and the increase was more pronounced at the late stage. Insulin treatment partially restored SO activity. However, the effect was temperature-dependent. In diabetic animals the energy of activation in the low temperature range (EL) increased significantly while that in the high temperature range (EH) decreased. The latter seems to be responsible for improving catalytic efficiency in the diabetic state. Insulin treatment normalized EH only in the 1-month diabetic group. The phase transition temperature (Tt), decreased in diabetic animals. Insulin treatment caused an increase beyond the control value in Tt in 1-month diabetic animals. The results suggest that insulin status-dependent modulation of SO activity is a complex process.
KW - Alloxan diabetes
KW - Arrhenius kinetics
KW - Diabetic modulation
KW - Insulin status
KW - Kidney mitochondria
KW - Succinate oxidase
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U2 - 10.1007/s00232-006-0041-2
DO - 10.1007/s00232-006-0041-2
M3 - Article
C2 - 17347780
AN - SCOPUS:33751242951
SN - 0022-2631
VL - 213
SP - 31
EP - 37
JO - Journal of Membrane Biology
JF - Journal of Membrane Biology
IS - 1
ER -
