Effect of bradykinin on NaCl transport in the medullary thick ascending limb of the rat

Jay Grider; Jeff Falcone; Eric Kilpatrick; Cobern Ott; Brian Jackson

doi:10.1016/0014-2999(95)00640-6

Effect of bradykinin on NaCl transport in the medullary thick ascending limb of the rat

Jay Grider
, Jeff Falcone
, Eric Kilpatrick
, Cobern Ott
, Brian Jackson

Research output: Contribution to journal › Article › peer-review

30 Scopus citations

Abstract

The aim of the present study was to determine whether bradykinin affects NaCl reabsorption in the medullary thick ascending limb of the loop of Henle. At 10^-8 M, bradykinin significantly inhibited Cl^- transport in the in vitro microperfused rat medullary thick ascending limb by 67% (P < 0.01). This inhibitory effect could be totally prevented by preincubating tubules with the bradykinin B₂ receptor antagonist Nα-adamantaneacetyl-d-Arg-[Hyp³,Thi^5,8,d-Phe⁷]bradykinin (10^-6 M). In contrast, the bradykinin B₁ receptor agonist des-Arg⁹ bradykinin (10^-6 M) had no effect on Cl^- transport. Bradykinin caused transient increases in intracellular Ca²⁺ concentration, which could be blocked by the bradykinin B₂ receptor antagonist, but could not be reproduced with the bradykinin B₁ receptor agonist. These data suggest that the natriuretic and diuretic effect of bradykinin in vivo is due, at least in part, to a bradykinin B₂ receptor-mediated inhibition of NaCl reabsorption in the medullary thick ascending limb of the loop of Henle.

Original language	English
Pages (from-to)	101-104
Number of pages	4
Journal	European Journal of Pharmacology
Volume	287
Issue number	1
DOIs	https://doi.org/10.1016/0014-2999(95)00640-6
State	Published - Dec 4 1995

Bibliographical note

Funding Information:
These studiesw ere supportedb y grants from the American Heart Association, Kentucky Affiliate (B.A.J.), NIH grant HL 53818 (J.C.F.), and NASA EPSCoR 522611( C.E.O.).

Funding

These studiesw ere supportedb y grants from the American Heart Association, Kentucky Affiliate (B.A.J.), NIH grant HL 53818 (J.C.F.), and NASA EPSCoR 522611( C.E.O.).

Funders	Funder number
Kentucky Affiliate
National Institutes of Health (NIH)
National Heart, Lung, and Blood Institute (NHLBI)	R29HL053818
National Aeronautics and Space Administration	522611
American Heart Association

Keywords

Bradykinin
Ca
Ion transport
Loop of Henle

ASJC Scopus subject areas

Pharmacology

Access to Document

10.1016/0014-2999(95)00640-6

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title = "Effect of bradykinin on NaCl transport in the medullary thick ascending limb of the rat",

abstract = "The aim of the present study was to determine whether bradykinin affects NaCl reabsorption in the medullary thick ascending limb of the loop of Henle. At 10-8 M, bradykinin significantly inhibited Cl- transport in the in vitro microperfused rat medullary thick ascending limb by 67\% (P < 0.01). This inhibitory effect could be totally prevented by preincubating tubules with the bradykinin B2 receptor antagonist Nα-adamantaneacetyl-d-Arg-[Hyp3,Thi5,8,d-Phe7]bradykinin (10-6 M). In contrast, the bradykinin B1 receptor agonist des-Arg9 bradykinin (10-6 M) had no effect on Cl- transport. Bradykinin caused transient increases in intracellular Ca2+ concentration, which could be blocked by the bradykinin B2 receptor antagonist, but could not be reproduced with the bradykinin B1 receptor agonist. These data suggest that the natriuretic and diuretic effect of bradykinin in vivo is due, at least in part, to a bradykinin B2 receptor-mediated inhibition of NaCl reabsorption in the medullary thick ascending limb of the loop of Henle.",

keywords = "Bradykinin, Ca, Ion transport, Loop of Henle",

author = "Jay Grider and Jeff Falcone and Eric Kilpatrick and Cobern Ott and Brian Jackson",

note = "Funding Information: These studiesw ere supportedb y grants from the American Heart Association, Kentucky Affiliate (B.A.J.), NIH grant HL 53818 (J.C.F.), and NASA EPSCoR 522611( C.E.O.).",

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AU - Grider, Jay

AU - Falcone, Jeff

AU - Kilpatrick, Eric

AU - Ott, Cobern

AU - Jackson, Brian

N1 - Funding Information: These studiesw ere supportedb y grants from the American Heart Association, Kentucky Affiliate (B.A.J.), NIH grant HL 53818 (J.C.F.), and NASA EPSCoR 522611( C.E.O.).

PY - 1995/12/4

Y1 - 1995/12/4

N2 - The aim of the present study was to determine whether bradykinin affects NaCl reabsorption in the medullary thick ascending limb of the loop of Henle. At 10-8 M, bradykinin significantly inhibited Cl- transport in the in vitro microperfused rat medullary thick ascending limb by 67% (P < 0.01). This inhibitory effect could be totally prevented by preincubating tubules with the bradykinin B2 receptor antagonist Nα-adamantaneacetyl-d-Arg-[Hyp3,Thi5,8,d-Phe7]bradykinin (10-6 M). In contrast, the bradykinin B1 receptor agonist des-Arg9 bradykinin (10-6 M) had no effect on Cl- transport. Bradykinin caused transient increases in intracellular Ca2+ concentration, which could be blocked by the bradykinin B2 receptor antagonist, but could not be reproduced with the bradykinin B1 receptor agonist. These data suggest that the natriuretic and diuretic effect of bradykinin in vivo is due, at least in part, to a bradykinin B2 receptor-mediated inhibition of NaCl reabsorption in the medullary thick ascending limb of the loop of Henle.

AB - The aim of the present study was to determine whether bradykinin affects NaCl reabsorption in the medullary thick ascending limb of the loop of Henle. At 10-8 M, bradykinin significantly inhibited Cl- transport in the in vitro microperfused rat medullary thick ascending limb by 67% (P < 0.01). This inhibitory effect could be totally prevented by preincubating tubules with the bradykinin B2 receptor antagonist Nα-adamantaneacetyl-d-Arg-[Hyp3,Thi5,8,d-Phe7]bradykinin (10-6 M). In contrast, the bradykinin B1 receptor agonist des-Arg9 bradykinin (10-6 M) had no effect on Cl- transport. Bradykinin caused transient increases in intracellular Ca2+ concentration, which could be blocked by the bradykinin B2 receptor antagonist, but could not be reproduced with the bradykinin B1 receptor agonist. These data suggest that the natriuretic and diuretic effect of bradykinin in vivo is due, at least in part, to a bradykinin B2 receptor-mediated inhibition of NaCl reabsorption in the medullary thick ascending limb of the loop of Henle.

KW - Bradykinin

KW - Ca

KW - Ion transport

KW - Loop of Henle

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Effect of bradykinin on NaCl transport in the medullary thick ascending limb of the rat

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

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