Effect of colonic bypass on diffuse mucosal inflammation in rats

F. A. Pon, B. R. MacPherson, S. K. Kilam

Research output: Contribution to journalArticlepeer-review


Colonic bypass achieved via split ileostomy or diverting ileostomy has been used since 1960 to facilitate numerous objectives, the main one being healing of affected segments. This study was designed to assess the effect of bowel rest through colonic bypass on the healing of experimentally inflamed descending colon in the rat. Two aspects in particular were examined: i) area of mucosa lesioned and ii) length of the mucosal crypts and number of epithelial cells per crypt. Seventy-two male Sprague-Dawley rats with an average weight of 250 g were used. Diffuse, nonspecific colitis was induced using the acetic acid method. Colonic bypass was established by dividing the ileum proximal to the ileocecal junction and anastomosing its proximal end to the lower region of the descending colon. 3 experimental groups were structured: i) animals with inflammation prior to bypass, ii) a control group (I) with inflamed mucosa but no bypass and iii) a second control group (II) that were not inflamed but received a bypass. Colonic bypass significantly reduced the area of colonic mucosa with visual lesions by the 7th day following surgery. There was a correlative beneficial effect with respect to the rate of mucosal healing when evaluated using a predetermined severity index. Mucosal inflammation was observed to cause an initial lengthening of the crypts by increasing the number of cells per crypt column. Colonic bypass brought about a return to near normal values much quicker than unbypassed animals when both were exposed to the inflammatory process. Animals with bypass but no inflammation experienced a reduction in crypt length from normal values effected through a reduction in the number of epithelial cells per crypt column.

Original languageEnglish
Pages (from-to)634-635
Number of pages2
JournalIRCS Medical Science
Issue number8
StatePublished - 1982

ASJC Scopus subject areas

  • General Biochemistry, Genetics and Molecular Biology


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