Effect of dexamethasone on voltage-gated Ca2+ channels and cytosolic Ca2+ in rat chromaffin cells

Lynn Fuller, Chengbiao Lu, Douglas McMahon, Elora Alaudin, Mark Jorgensen, Shane Rau, Jesse Sisken, Brian Jackson

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

The present study examined whether the synthetic glucocorticoid dexamethasone (DEX) can modulate voltage-gated Ca2+ channel (VGCC) activity, and as a consequence agonist-induced increases in cytosolic Ca2+, in cultured rat adrenal medullary chromaffin (RAMC) cells. Exposure to 1 μM DEX for 48 h significantly increased peak VGCC current (Δ +140%). DEX treatment also significantly potentiated the increases in cytosolic Ca2+ in response to submaximal stimulatory concentrations of KCl (Δ +64%) and nicotine (Δ +32%). The Ca2+ channel agonist BAY K-8644 increased both VGCC current (Δ +109%) and potentiated the KCl-stimulated increase in cytosolic Ca2+ (Δ +35%) to a comparable extent to that seen with DEX. These data suggest that DEX treatment increases VGCC activity, and that this increased Ca2+ influx leads to potentiation of agonist-induced increases in cytosolic Ca+ in RAMC cells.

Original languageEnglish
Pages (from-to)1169-1172
Number of pages4
JournalNeuroReport
Volume8
Issue number5
DOIs
StatePublished - 1997

Keywords

  • adrenal medulla
  • chromaffin cell
  • fura-2
  • glucocorticoids
  • intracellular calcium
  • voltage-gated calcium channel

ASJC Scopus subject areas

  • Neuroscience (all)

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