Effect of Dietary Vitamin D3 (Cholecalciferol) on Colon Carcinogenesis Induced by 1, 2-Dimethylhydrazine in Male Fischer 344 Rats

Patrick F. Comer; Terry D. Clark; Howard P. Glauert

doi:10.1080/01635589309514242

Effect of Dietary Vitamin D₃ (Cholecalciferol) on Colon Carcinogenesis Induced by 1, 2-Dimethylhydrazine in Male Fischer 344 Rats

Patrick F. Comer, Terry D. Clark, Howard P. Glauert

Pharmacology and Nutritional Sciences

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

This study examined the effect of increasing dietary vitamin D on chemically induced colon carcinogenesis. Male Fischer 344 rats were first injected with 1, 2-dimethylhydrazine (200 mg/kg) and then fed one of five dietary levels of vitamin D as cholecalciferol (250, 1,000, 2,000, 4,000, and 10,000 IU/kg diet) for nine months. Dietary vitamin D₃ had no effect on weight gain. Plasma 25-hydroxyvitamin D₃levels were similar for the 1,000 and 2,000 IU/kg groups but varied in a dose-related manner for the other groups. Vitamin D did not significantly alter the tumor incidence in either the distal or the proximal colon. No significant differences in the labeling index were found in either the proximal or the distal colon. Within the distal colon, the proliferative zone increased in a dose-related manner. Distribution of labeled cells within the crypt compartments was not affected by dietary vitamin D. Bone and serum minerals in general were unaffected by dietary vitamin D. This study shows that, at this level of dietary calcium, vitamin D did not affect 1, 2-dimethylhydrazine-induced colon carcinogenesis.

Original language	English
Pages (from-to)	113-124
Number of pages	12
Journal	Nutrition and Cancer
Volume	19
Issue number	2
DOIs	https://doi.org/10.1080/01635589309514242
State	Published - Jan 1 1993

Bibliographical note

Funding Information:
The authors thank Drs. Mary Stuart, Craig McClain, and Ramesh Talwalkar for help in the analysis of bone mineral content and Dr. Richard Kryscio for valuable statistical advice. This study was supported by American Institute for Cancer Research (Washington, DC) Grant No. 87B45. This is Kentucky Agricultural Experiment Station Article No. 91-9-50. Address reprint requests to Dr. Howard P. Glauert, University of Kentucky, Dept. of Nutrition and Food Science, 212 Funkhouser Bldg., Lexington, KY 40506-0054.

ASJC Scopus subject areas

Medicine (miscellaneous)
Oncology
Nutrition and Dietetics
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1080/01635589309514242

Cite this

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title = "Effect of Dietary Vitamin D3 (Cholecalciferol) on Colon Carcinogenesis Induced by 1, 2-Dimethylhydrazine in Male Fischer 344 Rats",

abstract = "This study examined the effect of increasing dietary vitamin D on chemically induced colon carcinogenesis. Male Fischer 344 rats were first injected with 1, 2-dimethylhydrazine (200 mg/kg) and then fed one of five dietary levels of vitamin D as cholecalciferol (250, 1,000, 2,000, 4,000, and 10,000 IU/kg diet) for nine months. Dietary vitamin D3 had no effect on weight gain. Plasma 25-hydroxyvitamin D3levels were similar for the 1,000 and 2,000 IU/kg groups but varied in a dose-related manner for the other groups. Vitamin D did not significantly alter the tumor incidence in either the distal or the proximal colon. No significant differences in the labeling index were found in either the proximal or the distal colon. Within the distal colon, the proliferative zone increased in a dose-related manner. Distribution of labeled cells within the crypt compartments was not affected by dietary vitamin D. Bone and serum minerals in general were unaffected by dietary vitamin D. This study shows that, at this level of dietary calcium, vitamin D did not affect 1, 2-dimethylhydrazine-induced colon carcinogenesis.",

author = "Comer, {Patrick F.} and Clark, {Terry D.} and Glauert, {Howard P.}",

note = "Funding Information: The authors thank Drs. Mary Stuart, Craig McClain, and Ramesh Talwalkar for help in the analysis of bone mineral content and Dr. Richard Kryscio for valuable statistical advice. This study was supported by American Institute for Cancer Research (Washington, DC) Grant No. 87B45. This is Kentucky Agricultural Experiment Station Article No. 91-9-50. Address reprint requests to Dr. Howard P. Glauert, University of Kentucky, Dept. of Nutrition and Food Science, 212 Funkhouser Bldg., Lexington, KY 40506-0054.",

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N1 - Funding Information: The authors thank Drs. Mary Stuart, Craig McClain, and Ramesh Talwalkar for help in the analysis of bone mineral content and Dr. Richard Kryscio for valuable statistical advice. This study was supported by American Institute for Cancer Research (Washington, DC) Grant No. 87B45. This is Kentucky Agricultural Experiment Station Article No. 91-9-50. Address reprint requests to Dr. Howard P. Glauert, University of Kentucky, Dept. of Nutrition and Food Science, 212 Funkhouser Bldg., Lexington, KY 40506-0054.

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N2 - This study examined the effect of increasing dietary vitamin D on chemically induced colon carcinogenesis. Male Fischer 344 rats were first injected with 1, 2-dimethylhydrazine (200 mg/kg) and then fed one of five dietary levels of vitamin D as cholecalciferol (250, 1,000, 2,000, 4,000, and 10,000 IU/kg diet) for nine months. Dietary vitamin D3 had no effect on weight gain. Plasma 25-hydroxyvitamin D3levels were similar for the 1,000 and 2,000 IU/kg groups but varied in a dose-related manner for the other groups. Vitamin D did not significantly alter the tumor incidence in either the distal or the proximal colon. No significant differences in the labeling index were found in either the proximal or the distal colon. Within the distal colon, the proliferative zone increased in a dose-related manner. Distribution of labeled cells within the crypt compartments was not affected by dietary vitamin D. Bone and serum minerals in general were unaffected by dietary vitamin D. This study shows that, at this level of dietary calcium, vitamin D did not affect 1, 2-dimethylhydrazine-induced colon carcinogenesis.

AB - This study examined the effect of increasing dietary vitamin D on chemically induced colon carcinogenesis. Male Fischer 344 rats were first injected with 1, 2-dimethylhydrazine (200 mg/kg) and then fed one of five dietary levels of vitamin D as cholecalciferol (250, 1,000, 2,000, 4,000, and 10,000 IU/kg diet) for nine months. Dietary vitamin D3 had no effect on weight gain. Plasma 25-hydroxyvitamin D3levels were similar for the 1,000 and 2,000 IU/kg groups but varied in a dose-related manner for the other groups. Vitamin D did not significantly alter the tumor incidence in either the distal or the proximal colon. No significant differences in the labeling index were found in either the proximal or the distal colon. Within the distal colon, the proliferative zone increased in a dose-related manner. Distribution of labeled cells within the crypt compartments was not affected by dietary vitamin D. Bone and serum minerals in general were unaffected by dietary vitamin D. This study shows that, at this level of dietary calcium, vitamin D did not affect 1, 2-dimethylhydrazine-induced colon carcinogenesis.

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