Effect of Dietary Vitamin D3 (Cholecalciferol) on Colon Carcinogenesis Induced by 1, 2-Dimethylhydrazine in Male Fischer 344 Rats

Patrick F. Comer, Terry D. Clark, Howard P. Glauert

Research output: Contribution to journalArticlepeer-review

17 Scopus citations


This study examined the effect of increasing dietary vitamin D on chemically induced colon carcinogenesis. Male Fischer 344 rats were first injected with 1, 2-dimethylhydrazine (200 mg/kg) and then fed one of five dietary levels of vitamin D as cholecalciferol (250, 1,000, 2,000, 4,000, and 10,000 IU/kg diet) for nine months. Dietary vitamin D3 had no effect on weight gain. Plasma 25-hydroxyvitamin D3levels were similar for the 1,000 and 2,000 IU/kg groups but varied in a dose-related manner for the other groups. Vitamin D did not significantly alter the tumor incidence in either the distal or the proximal colon. No significant differences in the labeling index were found in either the proximal or the distal colon. Within the distal colon, the proliferative zone increased in a dose-related manner. Distribution of labeled cells within the crypt compartments was not affected by dietary vitamin D. Bone and serum minerals in general were unaffected by dietary vitamin D. This study shows that, at this level of dietary calcium, vitamin D did not affect 1, 2-dimethylhydrazine-induced colon carcinogenesis.

Original languageEnglish
Pages (from-to)113-124
Number of pages12
JournalNutrition and Cancer
Issue number2
StatePublished - Jan 1 1993

Bibliographical note

Funding Information:
The authors thank Drs. Mary Stuart, Craig McClain, and Ramesh Talwalkar for help in the analysis of bone mineral content and Dr. Richard Kryscio for valuable statistical advice. This study was supported by American Institute for Cancer Research (Washington, DC) Grant No. 87B45. This is Kentucky Agricultural Experiment Station Article No. 91-9-50. Address reprint requests to Dr. Howard P. Glauert, University of Kentucky, Dept. of Nutrition and Food Science, 212 Funkhouser Bldg., Lexington, KY 40506-0054.

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Oncology
  • Nutrition and Dietetics
  • Cancer Research


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