Effect of endotoxin on lipid order and motion in erythrocyte membranes

D. Allan Butterfield; Bin Sun; Srithal Bellary; Warwick A. Arden; Kimberly Ward Anderson

doi:10.1016/0925-4439(94)90083-3

Effect of endotoxin on lipid order and motion in erythrocyte membranes

D. Allan Butterfield
, Bin Sun
, Srithal Bellary
, Warwick A. Arden
, Kimberly Ward Anderson

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

Electron paramagnetic resonance employing a lipid-specific spin label has been used to investigate the molecular effects of endotoxin on the physical state of bilayer lipids in rat erythrocyte membranes. When added at a concentration as low as 40 μg/ml to whole blood (plasma plus leukocytes present), decreased membrane lipid motion was found in subsequently washed and spin-labeled intact erythrocytes (P < 0.02). However, if endotoxin were added to washed, plasma plus leukocyte-free intact erythrocytes, no change in the motion of the spin label was found, suggesting that plasma-soluble substances and/or leukocytes are required to produce the change in the physical state of lipids. The decreased lipid motion found in these studies is discussed with reference to the known decreased deformability of endotoxin-treated red cells and to the pathogenesis of sepsis.

Original language	English
Pages (from-to)	231-234
Number of pages	4
Journal	Biochimica et Biophysica Acta - Molecular Basis of Disease
Volume	1225
Issue number	2
DOIs	https://doi.org/10.1016/0925-4439(94)90083-3
State	Published - Jan 11 1994

Bibliographical note

Funding Information:
This work was supported in part by grants from NSF \[EHR-9108764\] (D.A.B. and K.W.A.), \[CTS-9157856\] (K.W.A.) and NIH \[AG-10836\] (D.A.B.), \[HL-02918\] (W.A.A.).

Funding

This work was supported in part by grants from NSF \[EHR-9108764\] (D.A.B. and K.W.A.), \[CTS-9157856\] (K.W.A.) and NIH \[AG-10836\] (D.A.B.), \[HL-02918\] (W.A.A.).

Funders	Funder number
National Science Foundation (NSF)	EHR-9108764\, CTS-9157856\
National Institutes of Health (NIH)	AG-10836\
National Heart, Lung, and Blood Institute (NHLBI)	K08HL002918

Keywords

5-doxylstearic acid
Endotoxin
Lipid fluidity
Sepsis
Spin labeling

ASJC Scopus subject areas

Molecular Medicine
Molecular Biology

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10.1016/0925-4439(94)90083-3

Cite this

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title = "Effect of endotoxin on lipid order and motion in erythrocyte membranes",

abstract = "Electron paramagnetic resonance employing a lipid-specific spin label has been used to investigate the molecular effects of endotoxin on the physical state of bilayer lipids in rat erythrocyte membranes. When added at a concentration as low as 40 μg/ml to whole blood (plasma plus leukocytes present), decreased membrane lipid motion was found in subsequently washed and spin-labeled intact erythrocytes (P < 0.02). However, if endotoxin were added to washed, plasma plus leukocyte-free intact erythrocytes, no change in the motion of the spin label was found, suggesting that plasma-soluble substances and/or leukocytes are required to produce the change in the physical state of lipids. The decreased lipid motion found in these studies is discussed with reference to the known decreased deformability of endotoxin-treated red cells and to the pathogenesis of sepsis.",

keywords = "5-doxylstearic acid, Endotoxin, Lipid fluidity, Sepsis, Spin labeling",

author = "Butterfield, \{D. Allan\} and Bin Sun and Srithal Bellary and Arden, \{Warwick A.\} and Anderson, \{Kimberly Ward\}",

note = "Funding Information: This work was supported in part by grants from NSF \textbackslash{}[EHR-9108764\textbackslash{}] (D.A.B. and K.W.A.), \textbackslash{}[CTS-9157856\textbackslash{}] (K.W.A.) and NIH \textbackslash{}[AG-10836\textbackslash{}] (D.A.B.), \textbackslash{}[HL-02918\textbackslash{}] (W.A.A.).",

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T1 - Effect of endotoxin on lipid order and motion in erythrocyte membranes

AU - Butterfield, D. Allan

AU - Sun, Bin

AU - Bellary, Srithal

AU - Arden, Warwick A.

AU - Anderson, Kimberly Ward

N1 - Funding Information: This work was supported in part by grants from NSF \[EHR-9108764\] (D.A.B. and K.W.A.), \[CTS-9157856\] (K.W.A.) and NIH \[AG-10836\] (D.A.B.), \[HL-02918\] (W.A.A.).

PY - 1994/1/11

Y1 - 1994/1/11

N2 - Electron paramagnetic resonance employing a lipid-specific spin label has been used to investigate the molecular effects of endotoxin on the physical state of bilayer lipids in rat erythrocyte membranes. When added at a concentration as low as 40 μg/ml to whole blood (plasma plus leukocytes present), decreased membrane lipid motion was found in subsequently washed and spin-labeled intact erythrocytes (P < 0.02). However, if endotoxin were added to washed, plasma plus leukocyte-free intact erythrocytes, no change in the motion of the spin label was found, suggesting that plasma-soluble substances and/or leukocytes are required to produce the change in the physical state of lipids. The decreased lipid motion found in these studies is discussed with reference to the known decreased deformability of endotoxin-treated red cells and to the pathogenesis of sepsis.

AB - Electron paramagnetic resonance employing a lipid-specific spin label has been used to investigate the molecular effects of endotoxin on the physical state of bilayer lipids in rat erythrocyte membranes. When added at a concentration as low as 40 μg/ml to whole blood (plasma plus leukocytes present), decreased membrane lipid motion was found in subsequently washed and spin-labeled intact erythrocytes (P < 0.02). However, if endotoxin were added to washed, plasma plus leukocyte-free intact erythrocytes, no change in the motion of the spin label was found, suggesting that plasma-soluble substances and/or leukocytes are required to produce the change in the physical state of lipids. The decreased lipid motion found in these studies is discussed with reference to the known decreased deformability of endotoxin-treated red cells and to the pathogenesis of sepsis.

KW - 5-doxylstearic acid

KW - Endotoxin

KW - Lipid fluidity

KW - Sepsis

KW - Spin labeling

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AN - SCOPUS:0028144490

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JO - Biochimica et Biophysica Acta - Molecular Basis of Disease

JF - Biochimica et Biophysica Acta - Molecular Basis of Disease

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Effect of endotoxin on lipid order and motion in erythrocyte membranes

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

Access to Document

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