TY - JOUR
T1 - Effect of fatty acid on performance and lipid content of hearts from diabetic rabbits
AU - Fields, L. E.
AU - Daugherty, A.
AU - Bergmann, S. R.
PY - 1986
Y1 - 1986
N2 - The relationship of external work to exogenous substrate supply and whole heart lipid content was studied in hearts isolated from 27 normal and 24 alloxan-diabetic rabbits. In addition, the authors evaluated hearts from seven rabbits after 4 days of untreated diabetes followed by 10 days of insulin therapy. Hearts were retrogradely perfused with erythrocyte-enriched modified Krebs-Henseleit media in which glucose, palmitate, and insulin were present in concentrations simulating diabetic plasma (13 mM, 0.4 mM, and 15 μU/ml, respectively). Diabetes was associated with a >40% reduction in peak left ventricular systolic pressure, dP/dt(max), and left ventricular pressure-time index (P<0.01 for each). Perfusion of hearts from six diabetic animals with low concentrations of exogenous fatty acid (0.06 mM) normalized left ventricular (LV) performance. Insulin therapy also completely normalized LV performance. This improvement was associated with lower heart fatty acid and trialcyglycerol content. These studies suggest that the myocardial dysfunction in hearts from rabbits with alloxan-induced diabetes of 2-wk duration may be, in part, due to enhanced sensitivity to deleterious effects of high exogenous levels of fatty acid.
AB - The relationship of external work to exogenous substrate supply and whole heart lipid content was studied in hearts isolated from 27 normal and 24 alloxan-diabetic rabbits. In addition, the authors evaluated hearts from seven rabbits after 4 days of untreated diabetes followed by 10 days of insulin therapy. Hearts were retrogradely perfused with erythrocyte-enriched modified Krebs-Henseleit media in which glucose, palmitate, and insulin were present in concentrations simulating diabetic plasma (13 mM, 0.4 mM, and 15 μU/ml, respectively). Diabetes was associated with a >40% reduction in peak left ventricular systolic pressure, dP/dt(max), and left ventricular pressure-time index (P<0.01 for each). Perfusion of hearts from six diabetic animals with low concentrations of exogenous fatty acid (0.06 mM) normalized left ventricular (LV) performance. Insulin therapy also completely normalized LV performance. This improvement was associated with lower heart fatty acid and trialcyglycerol content. These studies suggest that the myocardial dysfunction in hearts from rabbits with alloxan-induced diabetes of 2-wk duration may be, in part, due to enhanced sensitivity to deleterious effects of high exogenous levels of fatty acid.
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U2 - 10.1152/ajpheart.1986.250.6.h1079
DO - 10.1152/ajpheart.1986.250.6.h1079
M3 - Article
C2 - 3521333
AN - SCOPUS:0022921430
SN - 0363-6135
VL - 250
SP - H1079-H1085
JO - American Journal of Physiology - Heart and Circulatory Physiology
JF - American Journal of Physiology - Heart and Circulatory Physiology
IS - 6 (19/6)
ER -