Effect of hypolipidemic peroxisome proliferators on unscheduled DNA synthesis in cultured hepatocytes and on mutagenesis in Salmonella

Howard P. Glauert, Janardan K. Reddy, Wendy S. Kennan, Gerald L. Sattler, V. Subba Rao, Henry C. Pitot

Research output: Contribution to journalArticlepeer-review

73 Scopus citations

Abstract

The peroxisome proliferators Wy-14,643, BR-931, nafenopin and ciprofibrate were tested in the primary hepatocyte culture-unscheduled DNA synthesis assay and in the Ames Salmonella microsome mutagenicity assay. The amount of unscheduled DNA synthesis (UDS) in hepatocytes was determined by quantifying the amount of [3H] thymidine incorporated into DNA in the presence of hydroxyurea after isolation of nuclei from hepatocytes treated with the test agent. Wy-14,643 and BR-931 induced unscheduled DNA synthesis in rat hepatocytes, whereas nafenopin and ciprofibrate had no effect. All of the peroxisome proliferators were negative in the Ames Salmonella assay.

Original languageEnglish
Pages (from-to)147-156
Number of pages10
JournalCancer Letters
Volume24
Issue number2
DOIs
StatePublished - Sep 1984

Bibliographical note

Funding Information:
This work was supported by DHHS PHS National Research Service Award 5 T32 ES07015 from the National Institute of Environmental Health Sciences, Grants CA-07175 and CA-22484 from the National Cancer Institute, and Grant GM-23750 from the National Institutes of Health.

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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