The peroxisome proliferators Wy-14,643, BR-931, nafenopin and ciprofibrate were tested in the primary hepatocyte culture-unscheduled DNA synthesis assay and in the Ames Salmonella microsome mutagenicity assay. The amount of unscheduled DNA synthesis (UDS) in hepatocytes was determined by quantifying the amount of [3H] thymidine incorporated into DNA in the presence of hydroxyurea after isolation of nuclei from hepatocytes treated with the test agent. Wy-14,643 and BR-931 induced unscheduled DNA synthesis in rat hepatocytes, whereas nafenopin and ciprofibrate had no effect. All of the peroxisome proliferators were negative in the Ames Salmonella assay.
|Number of pages
|Published - Sep 1984
Bibliographical noteFunding Information:
This work was supported by DHHS PHS National Research Service Award 5 T32 ES07015 from the National Institute of Environmental Health Sciences, Grants CA-07175 and CA-22484 from the National Cancer Institute, and Grant GM-23750 from the National Institutes of Health.
ASJC Scopus subject areas
- Cancer Research